Bioprinting of transparent and adhesive corneal patches: Integrating photo-crosslinkable dopamine-conjugated silk fibroin and decellularized cornea matrix for sutureless tissue integration and regeneration

Corneal injuries, a leading cause of visual impairment, are traditionally addressed through tissue transplantation. However, challenges such as donor shortages, graft rejection, and complications from suturing often limit their effectiveness. Current corneal adhesives frequently fall short in both adhesion strength and biocompatibility. We present an innovative solution: a photocurable hydrogel that integrates dopamine-conjugated methacrylated silk fibroin (d-MSF) with a decellularized corneal matrix (DCM). This hydrogel combines advanced materials to create a bioadhesive system that offers superior adhesion inspired by mussel adhesion and mimics the native tissue environment. FTIR and NMR analyses confirm that our conjugation process prevents unwanted beta-sheet aggregation, ensuring both stability and transparency. The hydrogel demonstrates excellent rheological properties, including enhanced shear-thinning and impressive shear and creep recovery, making it highly suitable for extrusion-based bioprinting. We successfully bioprinted a bilayer corneal patch, featuring a concentric ring of dMSF as the first layer, overlaid with a second layer of DCM. The implants exhibit strong tissue adhesion, with an adhesion strength of 85 +/- 5.6 KPa, and Young's modulus of 0.48 +/- 0.064 MPa, ensuring excellent structural integrity. This results in a highly transparent (>80 %) and functional adhesive corneal patch. This advancement offers a promising, biocompatible alternative to traditional keratoprostheses, advancing corneal repair technology.