Ergothioneine Stimulates Ca2+-Mediated Brain-Derived Neurotrophic Factor Expression in NE-4C Nerve Cells

被引：0

作者：

Shi, Caiyue ^{[1
]}

Asaba, Sumire ^{[1
]}

Nakamura, Saya ^{[1
]}

Matsui, Toshiro ^{[1
]}

机构：

[1] Kyushu Univ, Fac Agr, Grad Sch, Dept Biosci & Biotechnol, Fukuoka 8190395, Japan

来源：

ACS OMEGA | 2025年 / 10卷 / 07期

基金：

日本学术振兴会;

关键词：

OXIDATIVE STRESS; METABOLISM; RECEPTOR; BDNF;

D O I：

10.1021/acsomega.4c09920

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Ergothioneine (EGT), a naturally occurring histidine derivative, has been reported to modulate neurodegenerative diseases; however, the underlying mechanism remains unclear. This study aimed to investigate the brain-beneficial role of the natural amino acid EGT in NE-4C nerve cells. In the nerve cells, EGT treatment of >10 mu M for 48 h significantly increased the expression of brain-derived neurotrophic factor (BDNF), as well as the phosphorylation of cAMP response element-binding protein (CREB), whereas no change was observed in acetylcholine receptor expression. Additionally, EGT induced an increase in intracellular Ca2+ levels via stimulation of the inositol 1,4,5-triphosphate receptor (IP3R) in the endoplasmic reticulum; this increase was abrogated by the inhibition of organic cation transporter 1 (OCTN1). Structure-activity relationship analysis revealed the importance of the trimethylammonium group in EGT for intracellular events. In conclusion, EGT incorporated into cells via the OCTN1 route may act as a nerve transmission stimulator via IP3R-mediated Ca2+-CREB/BDNF activation.

引用

页码：7004 / 7012

页数：9