Correlation between antioxidant capacity and advanced glycation endproducts inhibition in enzyme-treated polysaccharides from Sargassum fusiforme

Marine polysaccharides demonstrate strong antioxidant capacity and can effectively alleviate oxidative stress. However, their high molecular weight often makes them resistant to enzymatic breakdown in the digestive system, limiting their bioavailability, and functional effectiveness. This study explored the potential of enzyme-treated polysaccharides derived from Sargassum fusiforme (EPS) for antioxidant and anti-glycation applications and elucidated their potential in managing oxidative stress. Polysaccharide from S. fusiforme was enzyme-treated using alpha-amylase, cellulase, and alpha-amylase derived from Caco-2 cells. EPSs generated were analyzed for molecular weight distribution and residual metal ion content such as arsenic. EPSs exhibit enhanced antioxidant capacities and advanced glycation end-product (AGEs) inhibition, highlighting their potential in addressing oxidative stress and AGEs-related diseases. Correlation and principal component analysis revealed that molecular weight reduction positively influenced the antioxidant capacity and AGE-breaking activities. These findings suggest that EPSs could serve as a natural therapeutic agents in functional foods and nutraceuticals, offering a promising approach for mitigating oxidative stress-induced health issues.