Immunological characteristics of bronchoalveolar lavage fluid and blood across connective tissue disease-associated interstitial lung diseases

被引：2

作者：

Hirano, Aiko ^{[1
]}

Sakashita, Aki ^{[1
]}

Fujii, Wataru ^{[1
]}

Bassler, Kevin ^{[2
]}

Tsuji, Taisuke ^{[3
]}

Kadoya, Masatoshi ^{[4
]}

Omoto, Atsushi ^{[4
]}

Hiraoka, Noriya ^{[3
]}

Imabayashi, Tatsuya ^{[5
]}

Kaneko, Yoshiko ^{[5
]}

Sofue, Hideaki ^{[1
]}

Maehara, Yosuke ^{[6
]}

Seno, Takahiro ^{[1
]}

Wada, Makoto ^{[1
]}

Kohno, Masataka ^{[1
]}

Fukuda, Wataru ^{[4
]}

Yamada, Kei ^{[6
]}

Takayama, Koichi ^{[5
]}

Kawahito, Yutaka ^{[1
]}

机构：

[1] Kyoto Prefectural Univ Med, Grad Sch Med Sci, Inflammat & Immunol, Kyoto, Japan

[2] Aimed Analyt GmbH, Bonn, Germany

[3] Japanese Red Cross Kyoto Daiichi Hosp, Dept Resp Med, Kyoto, Japan

[4] Japanese Red Cross Kyoto Daiichi Hosp, Ctr Rheumat Dis, Kyoto, Japan

[5] Kyoto Prefectural Univ Med, Grad Sch Med Sci, Dept Pulm Med, Kyoto, Japan

[6] Kyoto Prefectural Univ Med, Grad Sch Med Sci, Dept Radiol, Kyoto, Japan

来源：

FRONTIERS IN IMMUNOLOGY | 2024年 / 15卷

关键词：

single-cell RNA sequencing; genetics; interstitial lung disease; connective tissue disease; systemic autoimmune rheumatic disease; RHEUMATOLOGY CLASSIFICATION CRITERIA; 2022; AMERICAN-COLLEGE; ENDOPLASMIC-RETICULUM STRESS; SYSTEMIC-SCLEROSIS; SJOGRENS-SYNDROME; CELLS; LEAGUE; ALLIANCE; GRANULOMATOSIS; PATHOGENESIS;

D O I：

10.3389/fimmu.2024.1408880

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Interstitial lung disease (ILD) is a serious complication of connective tissue diseases (CTDs). The heterogeneity of ILDs reflects differences in pathogenesis among diseases. This study aimed to clarify the characteristics of CTD-ILDs via a detailed analysis of the bronchoalveolar lavage fluid (BALF) and blood immune cells. BALF and blood samples were collected from 39 Japanese patients with newly diagnosed ILD: five patients with Sj & ouml;gren's syndrome (SS), eight patients with dermatomyositis (DM), six patients with rheumatoid arthritis (RA), six patients with systemic sclerosis, four patients with anti-neutrophil cytoplasmic antibody-associated vasculitis, and 10 patients with idiopathic interstitial pneumonia. We performed single-cell RNA sequencing to analyze the gene expression profiles in these patients' immune cells. In patients with SS, B cells in the BALF were increased and genes associated with the innate and acquired immunity were enriched in both the BALF and blood. In contrast, patients with DM showed an upregulation of genes associated with viral infection in both the BALF and blood. In patients with RA, neutrophils in the BALF tended to increase, and their gene expression patterns changed towards inflammation. These disease-specific characteristics may help us understand the pathogenesis for each disease and discover potential biomarkers.

引用

页数：19

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