Harnessing high-level hydrogen sulfide stress for enhanced biogas utilization: Adaptive resilience of a mixed-culture system

被引：0

作者：

Zhang, Baorui ^{[1
,2
]}

Liu, Jianbo ^{[4
]}

Cai, Chen ^{[3
]}

Zhou, Yan ^{[2
,4
]}

机构：

[1] Nanyang Technol Univ, Interdisciplinary Grad Program, 61 Nanyang Dr, Singapore 637335, Singapore

[2] Nanyang Technol Univ, Nanyang Environm & Water Res Inst, 1 Cleantech Loop, Singapore 637141, Singapore

[3] Univ Sci & Technol China, Dept Environm Sci & Engn, CAS Key Lab Urban Pollutant Convers, Hefei, Peoples R China

[4] Nanyang Technol Univ, Sch Civil & Environm Engn, 50 Nanyang Ave, Singapore 639798, Singapore

来源：

CHEMICAL ENGINEERING JOURNAL | 2025年 / 506卷

关键词：

Biogas utilization; Hydrogen sulfide stress and response; Microbial community shift; Mixed-culture system; SP NOV; GEN; NOV; OXIDATION; METHANE; WATER; LEPTOLYNGBYA; TOLERANCE; BACTERIA; CARBON; ALGAE;

D O I：

10.1016/j.cej.2025.160300

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Biogas, a mixture predominantly composed of methane (CH4) and carbon dioxide (CO2), serves as a substrate for diverse microorganisms, including methanotrophs and microalgae, facilitating carbon sequestration, mitigating greenhouse gas emissions, and generating biomass for multifarious applications. However, the presence of hydrogen sulfide (H2S), a common contaminant in biogas, can exert toxic effects on these microorganisms, potentially inhibiting their growth and metabolic processes. This study investigated the effects of H2S on a mixed-culture system utilizing a long-term continuous flow photobioreactor. The results demonstrated that the mixed-culture system could tolerate H2S concentration up to 1000 ppm without significant performance deterioration. Notably, when the mixed-culture system was exposed to a higher H2S concentration (5000 ppm) for an extended period of one month, the CH4 and CO2 utilization rates increased threefold. Furthermore, the primary byproduct, protein, constituted over 50 % of the cell dry weight. Comprehensive analysis of the microbial community composition and metabolic pathways revealed adaptive shifts in response to H2S stress. In-depth analysis of functional genes indicated that the system possessed the capacity to adapt and maintain its functionality even under severe H2S stress. This study provides valuable insights into microbial and metabolic mechanisms underlying H2S resistance in a mixed culture, providing an opportunity for the establishment of an H2S-resistant biosystem for practical biogas transformation.

引用

页数：11