A Novel Formulation Based on Resveratrol and Water Extracts from Equisetum arvense, Crataegus curvisepala, Vitex agnus-castus, and Glycine max Inhibits the Gene Expression of Inflammatory and Osteoclastogenic Biomarkers on C2C12 Cells Exposed to Oxidative Stress

被引：0

作者：

Di Simone, Simonetta Cristina ^{[1
]}

Acquaviva, Alessandra ^{[1
]}

Libero, Maria Loreta ^{[1
]}

Nilofar, Nilofar ^{[1
]}

Tunali, Fatma ^{[1
]}

Gabriele, Mariachiara ^{[1
]}

Centulio, Angelica Pia ^{[1
]}

Genovesi, Gianluca ^{[1
]}

Ciaramellano, Davide ^{[1
]}

Recinella, Lucia ^{[1
]}

Leone, Sheila ^{[1
]}

Brunetti, Luigi ^{[1
]}

Zengin, Gokhan ^{[2
]}

Orlando, Giustino ^{[1
]}

Menghini, Luigi ^{[1
]}

Chiavaroli, Annalisa ^{[1
]}

Ferrante, Claudio ^{[1
]}

机构：

[1] Univ G dAnnunzio, Dept Pharm, Bot Garden Giardino dei Semplici, I-66100 Chieti, Italy

[2] Selcuk Univ, Sci Fac, Dept Biol, Physiol & Biochem Lab, TR-42130 Konya, Turkiye

来源：

FOODS | 2025年 / 14卷 / 05期

关键词：

Equisetum arvense; Crataegus curvisepala; Vitex agnus-castus; Glycine max; phenolic compounds; menopause; oxidative stress; OSTEOPOROSIS; ESTRADIOL;

D O I：

10.3390/foods14050896

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Medicinal plants and natural compounds have been considered alternative therapeutic options for counteracting postmenopausal disorders thanks to their different concomitant effects, including antioxidant and anti-inflammatory properties and the regulation of hormone activity. It is important to highlight that the efficacy of medicinal plants and natural compounds increases when used in combination, thus making the development of herbal formulations rational. Therefore, the present study aimed to evaluate the phytochemical and pharmacological properties of an innovative formulation consisting of resveratrol and water extracts from Equisetum arvense, Crateagus curvisepala, Vitex agnus-castus, and Glycine max. The phenolic composition and radical scavenger properties were evaluated using chromatographic and colorimetric (ABTS) methods, whilst the limits of biocompatibility were assessed through allelopathy, the Artemia salina (brine shrimp) lethality test, and Daphnia magna cardiotoxicity assay. The protective effects were evaluated on C2C12 cell lines exposed to the pro-oxidant stimulus, which consisted of hydrogen peroxide. The gene expression of estrogen 1 (ESR1, also known as ER alpha) and prolactin (PRLR) receptors, interleukin 6 (IL-6), tumor necrosis factor alpha (TNF alpha), and receptor activator of nuclear factor kappa-Beta ligand (RANKL) was measured. The results of the phytochemical analysis showed that the main phytochemicals were hydroxycinnamic and phenolic acids, in particular coumaric acid (7.53 mu g/mL) and rosmarinic acid (6.91 mu g/mL), respectively. This could explain the radical scavenger effect observed from the 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay. According to the ecotoxicological models' results, the formulation was revealed to be non-toxic, with a LC50 value > 1 mg/mL. Therefore, a biocompatible concentration range (200-1000 mu g/mL) was used in C2C12 cells, where the formulation blunted the hydrogen peroxide-induced upregulation of TNF alpha, IL-6, RANKL, ESR1, and PRLR. Overall, the results of this study corroborate the use of the formulation for facing the oxidative stress and inflammation, which forms the basis of the osteoclastogenic process.

引用

页数：20