Protocol for identifying sound-activated neurons in the inferior colliculus by cFos immunostaining

被引：0
|
作者：
Maio, Beatriz [1 ,2 ]
Elu, Nagore [1 ,2 ]
Martinez-Gonzalez, Cristina [1 ,2 ]
Osterweil, Emily K. [1 ,2 ,3 ]
Louros, Susana R. [1 ,2 ]
机构：
[1] Univ Edinburgh, Ctr Discovery Brain Sci, Edinburgh EH8 9XD, Scotland
[2] Univ Edinburgh, Patrick Wild Ctr, Simons Initiat Developing Brain, Edinburgh, Scotland
[3] Harvard Med Sch, Boston Childrens Hosp, Rosamund Stone Zander Translat Neurosci Ctr, FM Kirby Ctr,Dept Neurol, Boston, MA 02115 USA
来源：
STAR PROTOCOLS | 2024年 / 5卷 / 04期
关键词：
D O I：
10.1016/j.xpro.2024.103482
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
cfos is an immediate early gene commonly used to identify neuronal activation. After loud sound stimulation, neurons in the inferior colliculus are activated and cFos is expressed in the nucleus. Here, we present a protocol for quantifying neuronal activity in response to auditory stimulation using cFos immunostaining in the mouse inferior colliculus. We then detail procedures for image acquisition and analysis. For complete details on the use and execution of this protocol, please refer to Louros et al.1
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页数：18
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