The review summarizes the recent research progress of pressure-temperature dual-functional flexible sensors, including the sensing mechanisms, the design of dual-functional sensors, and their applications in biomedicine. The sensing mechanisms of the sensors include electrical signal mechanisms (resistance, voltage, and capacitance mechanisms) and non-electrical signal mechanisms (optical and magnetic mechanisms). Then, the design strategies of pressure-temperature dual-functional flexible sensors are discussed, including the integration of different types of sensors and the design of single dual-functional sensors composed of multifunctional materials and microstructures. Finally, the applications of pressure-temperature dual-functional flexible sensors in human motion detection, health monitoring, artificial intelligence, and human-machine interaction are introduced. Based on this, the problems of dual-functional flexible sensors are summarized, and the future development prospects are discussed.