A Rapid and Sensitive SERS-Based Au/MIL-125(Ti) Biosensor for Hyperuricemia Diagnosis via Saliva Analysis

An Au/MIL-125(Ti) nanocomposite was developed as a novel Raman-based biosensor platform for the noninvasive evaluation of uric acid (UA) levels in saliva. The intensity ratio (I 497/I 1648) for the Raman spectrum of uric acid was found to exhibit a quantitative correlation with uric acid concentration upon its adsorption onto MIL-125(Ti). This innovative method achieves an impressive limit of detection (LOD) of 0.01 mu M with consistent performance in physiological media. This platform shows a wide linear detection range from 50 mu M to 5000 mu M, showcasing its sensitivity and versatility. Furthermore, this biosensor facilitates rapid UA detection in saliva samples and can distinguish clearly hyperuricemia sufferers from healthy controls within a remarkable time frame of only 90 s without complicated pretreatments, highlighting its great potential for real-time diagnostics. This research underscores the transformative potential of composite biosensors based on metal-organic frameworks (MOFs) for biomarker sensing applications, paving the way for future innovations in noninvasive diagnostic technologies.