Diacylglycerol kinase δ is required for skeletal muscle development and regeneration

被引:0
|
作者
Sakai, Hiromichi [1 ]
Murakami, Chiaki [2 ,3 ]
Takechi, Mayumi [4 ]
Urano, Takeshi [5 ]
Sakane, Fumio [2 ]
机构
[1] Shimane Univ, Interdisciplinary Ctr Sci Res Org Res & Acad Infor, Dept Biosignaling & Radioisotope Expt, Izumo 6938501, Japan
[2] Chiba Univ, Grad Sch Sci, Dept Chem, Chiba 2638522, Japan
[3] Chiba Univ, Inst Adv Acad Res, Chiba, Japan
[4] Shimane Univ, Interdisciplinary Ctr Sci Res Org Res & Acad Infor, Dept Expt Anim, Izumo, Japan
[5] Shimane Univ, Sch Med, Dept Biochem, Izumo, Japan
基金
日本学术振兴会; 日本科学技术振兴机构;
关键词
diacylglycerol kinase; glucose tolerance; muscle development; muscle regeneration; satellite cell; type II diabetes; PHOSPHATIDIC-ACID; DOWN-REGULATION; GLUCOSE; EXPRESSION; ACTIVATION; INSULIN; HYPERGLYCEMIA; ISOFORMS; ETA;
D O I
10.1096/fba.2024-00134
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Diacylglycerol kinase delta (DGK delta) phosphorylates diacylglycerol to produce phosphatidic acid. Previously, we demonstrated that down-regulation of DGK delta suppresses the myogenic differentiation of C2C12 myoblasts. However, the myogenic roles of DGK delta in vivo remain unclear. In the present study, we generated DGK delta-conditional knockout mice under the control of the myogenic factor 5 (Myf5) gene promoter, which regulates myogenesis and brown adipogenesis. The knockout mice showed a significant body weight reduction and apparent mass decrease in skeletal muscle, including the tibialis anterior (TA) muscle. Moreover, the thickness of a portion of the myofibers was reduced in DGK delta-deficient TA muscles. However, DGK delta deficiency did not substantially affect brown adipogenesis, suggesting that Myf5-driven DGK delta deficiency mainly affects muscle development. Notably, skeletal muscle injury induced by a cardiotoxin highly up-regulated DGK delta protein expression, and the DGK delta deficiency significantly reduced the thickness of myofibers, the expression levels of myogenic differentiation markers such as embryonic myosin heavy chain and myogenin, and the number of newly formed myofibers containing multiple central nuclei during muscle regeneration. DGK delta was strongly expressed in myogenin-positive satellite cells around the injured myofibers and centronucleated myofibers. These results indicate that DGK delta has important roles in muscle regeneration in activated satellite cells. Moreover, the conditional knockout mice fed with a high-fat diet showed increased fat mass and glucose intolerance. Taken together, these results demonstrate that DGK delta plays crucial roles in skeletal muscle development, regeneration, and function.
引用
收藏
页数:14
相关论文
共 50 条
  • [1] uPAR is not required for skeletal muscle regeneration
    Bryer, SC
    Koh, TJ
    FASEB JOURNAL, 2006, 20 (04): : A803 - A803
  • [2] Skeletal muscle development and regeneration
    Grefte, Sander
    Kuijpers-Jagtman, Anne Marie
    Torensma, Ruurd
    Von den Hoff, Johannes W.
    STEM CELLS AND DEVELOPMENT, 2007, 16 (05) : 857 - 868
  • [3] BAMBI shuttling between cytosol and membrane is required for skeletal muscle development and regeneration
    Yao, Xiangping
    Yu, Taiyong
    Xi, Fengxue
    Xu, Yanting
    Ma, Lu
    Pan, Xiaohan
    Chen, Shujie
    Han, Mulan
    Yin, Yulong
    Dai, Xiaoshuang
    Xu, Guohuan
    Zhang, Huabing
    Yang, Gongshe
    Xie, Liwei
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2019, 509 (01) : 125 - 132
  • [4] Sox15 is required for skeletal muscle regeneration
    Lee, HJ
    Göring, W
    Ochs, M
    Mühlfeld, C
    Steding, G
    Paprotta, I
    Engel, W
    Adham, IM
    MOLECULAR AND CELLULAR BIOLOGY, 2004, 24 (19) : 8428 - 8436
  • [5] Commensal bacteria are required for the regeneration of damaged skeletal muscle
    Matsuo, Kaori
    Fujita, Motoo
    Nagatomi, Ryoichi
    FASEB JOURNAL, 2010, 24
  • [6] From development to regeneration of skeletal muscle
    Tajbakhsh, Shahragim
    Relaix, Frederic
    BIOFUTUR, 2011, (321) : 50 - 52
  • [7] Trbp Is Required for Differentiation of Myoblasts and Normal Regeneration of Skeletal Muscle
    Ding, Jian
    Nie, Mao
    Liu, Jianming
    Hu, Xiaoyun
    Ma, Lixin
    Deng, Zhong-Liang
    Wang, Da-Zhi
    PLOS ONE, 2016, 11 (05):
  • [8] PPARα is required for angiogenesis and tissue regeneration in the injured skeletal muscle
    Giarretta, Igor
    Neri, Valentina
    Gatto, Ilaria
    Palladino, Mariangela
    Biscetti, Federico
    Piccioni, Andrea
    Pola, Roberto
    VASCULAR PHARMACOLOGY, 2012, 56 (5-6) : 309 - 309
  • [9] Endogenous interferon-γ is required for efficient skeletal muscle regeneration
    Cheng, Ming
    Nguyen, Mai-Huong
    Fantuzzi, Giamila
    Koh, Timothy J.
    AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2008, 294 (05): : C1183 - C1191
  • [10] Wnt7a is required for regeneration of dystrophic skeletal muscle
    Gurriaran-Rodriguez, Uxia
    Kodippili, Kasun
    Datzkiw, David
    Javandoost, Ehsan
    Xiao, Fan
    Rejas, Maria Teresa
    Rudnicki, Michael A.
    SKELETAL MUSCLE, 2024, 14 (01):