Debut of enzyme-responsive anionic cyanine for overlap-free NIR-II-to-I dual-channel tumour imaging

被引：0

作者：

Chu, Feiyi ^{[1
]}

Feng, Bin ^{[1
]}

Zhou, Yiyang ^{[1
]}

Liu, Min ^{[1
,2
]}

Zhang, Hailiang ^{[1
]}

Liu, Meihui ^{[1
]}

Chen, Qian ^{[3
]}

Zhang, Shengwang ^{[3
]}

Ma, Yeshuo ^{[3
]}

Dong, Jie ^{[1
]}

Chen, Fei ^{[1
]}

Zeng, Wenbin ^{[1
]}

机构：

[1] Cent South Univ, Xiangya Sch Pharmaceut Sci, Changsha 410078, Peoples R China

[2] Xiangya Hosp, Dept Pharm, Changsha 410008, Peoples R China

[3] Cent South Univ, Xiangya Hosp 3, Changsha 410013, Peoples R China

来源：

CHEMICAL SCIENCE | 2025年 / 16卷 / 10期

基金：

中国国家自然科学基金;

关键词：

GAMMA-GLUTAMYL-TRANSPEPTIDASE; PROBES; GLUTATHIONE; CYSTEINE;

D O I：

10.1039/d4sc06459c

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Bridging the disparity between traditional surgical resection imaging and ex vivo histopathology, fluorescence imaging is considered a promising tool in disease diagnosis and imaging navigation. Nevertheless, its usefulness is undermined by the variability of single-wavelength fluorescence signals and limited penetration of NIR-I (650-900 nm) bioimaging. In this work, we present a novel NIR-II ratiometric fluorescent probe (CFC-GSH) with gamma-glutamyl transpeptidase (GGT) sensitivity for multifunctional bioimaging. This probe leverages a GSH-capped anionic cyanine, with advantages of high brightness, excellent photostability, high specificity and favourable biocompatibility. CFC-GSH exhibits an intrinsically stable NIR-II signal prior to triggering, which can be utilized for in vivo systemic circulation vessel outlining and microvascular imaging. At the tumour site with GGT over expression, an intramolecular S,N-rearrangement would initiate the conversion of sulphur-substituted cyanine to amino-substituted cyanine, resulting in a significant emission shift of 270 nm. Using the dual-channel signal changes, CFC-GSH effectively differentiates between subcutaneous hepatocellular carcinoma (HCC) and normal tissue and precisely localizes metastatic HCC tumours in the abdominal cavity. These results reveal that CFC-GSH exhibits promising potential as a multiprospective candidate tool for fluorescence screening and diagnostic imaging in various biological scenarios.

引用

页码：4490 / 4500

页数：11