Hydrogel integrating CBEO-CINcocktail (Cinnamomum pauciflorum Nees bark oil enriched with cinnamaldehyde) loaded nanosponges exhibited potent antibacterial activity against carbapenem resistant bacterial pathogens- in vitro and in vivo evaluation

被引：0

作者：

Das Mitra, Susweta ^{[1
]}

Shailaja, S. ^{[1
]}

Devi, R. Aruna ^{[1
]}

Mitra, Jyotibrata ^{[2
]}

Reddy, Navya R. ^{[1
]}

Ameera ^{[1
]}

Anshul, Pedda Marri ^{[1
]}

Harshitha, N. ^{[1
]}

Saha, Adrija ^{[1
]}

Jenita, J. Josephine Leno ^{[3
]}

Manjula, D. ^{[3
]}

More, Sunil S. ^{[1
]}

机构：

[1] Dayananda Sagar Univ, Sch Basic & Appl Sci, Dept Biotechnol, SBAS, Bangalore 560078, Karnataka, India

[2] Flower Valley Agro Tech Pvt Ltd AJMAL, Hojai, Assam, India

[3] Dayananda Sagar Univ, Coll Pharmaceut Sci, Bangalore 560078, Karnataka, India

来源：

INDUSTRIAL CROPS AND PRODUCTS | 2025年 / 225卷

关键词：

Cinnamomum pauciflorum Nees; Essential oil; Cocktail; Nanosponge; Hydrogel; Carbapenem resistant bacteria; FORMULATION; NANOPARTICLES;

D O I：

10.1016/j.indcrop.2025.120503

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Antibiotic resistance is increasingly hindering the conventional treatment of bacterial infections, including those related to surgery, implants, wounds, soft tissues etc. Essential oils and their bioactive compounds are emerging as promising alternatives to combat this escalating threat. In this study, we extracted essential oil (PC1-CBEO) from the mature barks of Cinnamomum pauciflorum Nees grown in Meghalaya, Northeast India. Gas Chromatography-Mass Spectrometry (GC-MS) analysis identified cinnamaldehyde (50.53 %) as the primary compound, followed by beta-caryophyllene (12 %). We purified cinnamaldehyde (PC2-CIN) and combined with PC1 to create a CBEO-CINcocktail (PCK), which demonstrated the lowest minimum inhibitory concentration (MIC) (0.03125-0.5 mg/ml) against carbapenem-resistant bacteria (E. coli, K. pneumoniae, A. baumannii, and P. aeruginosa), compared to PC1 (0.25-1 mg/ml) and PC2 (0.0625-1 mg/ml). The CBEO-CINcocktail exhibited a synergistic effect when combined with antibiotics, highlighting its potential as an adjuvant to enhance effectiveness of conventional antibiotics against multidrug-resistant (MDR) pathogens. In the second phase of the study, the CBEO-CINcocktail (PCK) was encapsulated in nanosponge carrier using a solvent emulsification method with ethyl cellulose and polyvinyl alcohol, forming PCK-NS3. The optimized nanosponges (PCK-NS3) exhibited favourable properties, including ideal particle size (350 nm), drug loading, and entrapment efficiency. When incorporated into a 0.5 % carbopol gel, the release kinetics followed the Higuchi model (R2=0.97), indicating controlled release. In a murine infection model, the gel demonstrated rapid lesion closure (99.58 +/- 0.12 % contraction) and reduced bacterial load compared to the untreated group (42.58 +/- 1.94 % contraction). The cocktail nanosponge gel demonstrated significant antibacterial activity against carbapenem-resistant bacterial infections in vitro and in vivo highlighting its therapeutic potential. Overall, this research study offers an essential oil based ecofriendly and sustainable antibacterial solution with broad applications for treating bacterial infections in both clinical and veterinary settings (companion animals and livestock). The observed effectiveness further opens up substantial prospects for using these natural agents as alternatives to reduce antibiotic growth promoters (AGP) in animal husbandry, decrease antibiotic use in animal farming, and safeguard one health (human, animal and environment).

引用

页数：17