Polydopamine-modified hydroxyapatite and manganese tetroxide nanozyme incorporated gelatin methacryloyl hydrogel: A multifunctional platform for anti-bacteria, immunomodulation, angiogenesis, and enhanced regeneration in infected wounds

被引：0

作者：

Cheng, Liang ^{[1
,2
]}

Zhang, Huan ^{[4
]}

Zhou, Bingshuai ^{[2
]}

Wang, Huaiwu ^{[5
]}

Sun, Yue ^{[2
,3
]}

Pang, Yuxuan ^{[1
]}

Dong, Biao ^{[2
]}

机构：

[1] Jilin Univ, Sch & Hosp Stomatol, Dept Prosthodont, Changchun 130021, Peoples R China

[2] Jilin Univ, Coll Elect Sci & Engn, State Key Lab Integrated Optoelect, Changchun 130021, Peoples R China

[3] Jilin Univ, Sch & Hosp Stomatol, Dept Implantol, Changchun 130021, Peoples R China

[4] Chinese Univ Hong Kong, Fac Med, Sch Biomed Sci, Hong Kong 999077, Peoples R China

[5] Changchun Inst Technol, Changchun 130022, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2025年 / 307卷

关键词：

Hydrogel; Anti-bacterial; Immunomodulatory; Angiogenesis; Infected wound healing; CELL; ANTIBACTERIAL; BIOMATERIALS; STIMULATION; DESIGN;

D O I：

10.1016/j.ijbiomac.2025.141834

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Intensive inflammation induced via bacterial infection seriously disturbs the immune-microenvironment and compromise the neovascularization in the skin wound. On the basis of reducing bacterial infections, alleviating inflammatory response and rebuild the crosstalk between macrophages and vascular endothelial cell (VEC) serve as the key strategy for facilitating infected wound healing. Herein, manganese tetroxide (Mn3O4) nanozymes and polydopamine-coated hydroxyapatite (PHA) nanoparticles were loaded on the gelatin methacryloyl (GelMA) hydrogel, which was subsequently crosslinked by the UV light to construct a multifunctional hydrogel wound dressing GelMA-PHA-Mn3O4 with excellent anti-bacterial, immuno-regulation and angiogenic properties. Triggered by near infrared (NIR), PHA exhibited photothermal effect and effectively eradicated Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) biofilm. On the other hand, Mn3O4 nanozymes in hydrogel exhibit desirable reactive oxygen species (ROS) scavenging capacity due to the redox cycle between Mn2+ and Mn3+, which successfully transform the LPS-induced macrophage phenotype from pro-inflammation M1 to anti- inflammation M2. Notably, the interaction between macrophages and VECs was subsequently reconstructed and exhibited an evident pro-angiogenic phenomenon along with the improvement of local immunomicroenvironment. In vivo study further verified that the GelMA-PHA-Mn3O4 hydrogel combined with NIR irradiation could accelerate the healing of infected wound through the prominent anti-bacterial and immunoregulation effect. The collagen deposition and formation of blood vessel in the wound were active. Above, this study demonstrated that the GelMA-PHA-Mn3O4 hydrogel represents a promising approach for managing infected wounds, with an anticipated prospect in clinical application.

引用

页数：16