Genome-Led Discovery of the Antibacterial Cyclic Lipopeptide Kutzneridine A and Its Silent Biosynthetic Gene Cluster from Kutzneria Species

被引：1

作者：

Ortiz-Lopez, Francisco Javier ^{[1
]}

Oves-Costales, Daniel ^{[1
]}

Garzon, Jaime Felipe Guerrero ^{[2
]}

Gren, Tetiana ^{[2
]}

Sterndorff, Eva Baggesgaard ^{[2
]}

Jiang, Xinglin ^{[2
]}

Jorgensen, Tue Sparholt ^{[2
]}

Blin, Kai ^{[2
]}

Fernandez-Pastor, Ignacio ^{[1
]}

Tormo, Jose R. ^{[1
]}

Martin, Jesus ^{[1
]}

Sanchez, Pilar ^{[1
]}

Moreno, Mercedes de la Cruz ^{[1
]}

Reyes, Fernando ^{[1
]}

Genilloud, Olga ^{[1
]}

Weber, Tilmann ^{[2
]}

机构：

[1] Fdn MEDINA, Ctr Excelencia Invest Medicamentos Innovadores And, Granada 18016, Spain

[2] Tech Univ Denmark, Novo Nordisk Fdn, Ctr Biosustainabil, DK-2800 Lyngby, Denmark

来源：

JOURNAL OF NATURAL PRODUCTS | 2024年 / 87卷 / 10期

关键词：

NATURAL-PRODUCTS; PEPTIDE; CHONBURIENSIS; ANTIBIOTICS; MECHANISM;

D O I：

10.1021/acs.jnatprod.4c00633

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Genome analysis of Kutzneria sp. CA-103260 revealed a putative lipopeptide-encoding biosynthetic gene cluster (BGC) that was cloned into a bacterial artificial chromosome (BAC) and heterologously expressed in Streptomyces coelicolor M1152. As a result, a novel cyclic lipo-tetrapeptide containing two diaminopropionic acid residues and an exotic N,N-acetonide ring, kutzneridine A (1), was isolated and structurally characterized. Evaluation of the extraction conditions and isotope-labeling chemical modifications showed that the acetonide ring originated from acetone during isolation. The BGC was analyzed in silico and a biosynthetic pathway to 1 was proposed. Kutzneridine A displayed remarkable antibacterial activity against methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococci.

引用

页码：2515 / 2522

页数：8

共 8 条

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