Anti-androgenetic effect of diphlorethohydroxycarmalol on testosterone-induced hair loss by inhibiting 5a-reductase and promoting Wnt/(3-catenin signaling pathway in human dermal papilla cells

被引：0

作者：

Im, Seung Tae ^{[1
]}

Mun, Haeun ^{[2
]}

Kang, Nalae ^{[3
]}

Heo, Soo-Jin ^{[3
]}

Lee, Seung-Hong ^{[4
]}

机构：

[1] Korea Food Res Inst, Res Grp Proc Engn, Wanju 55365, South Korea

[2] Beautyence Ltd, R&DB Ctr, 220 Sandan Gil, Sejong 30003, South Korea

[3] Korea Inst Ocean Sci & Technol, Jeju Marine Res Ctr, Jeju 63349, South Korea

[4] Soonchunhyang Univ, Dept Med Sci & Pharmaceut Engn, Asan 31538, South Korea

来源：

TOXICOLOGY IN VITRO | 2025年 / 104卷

关键词：

Diphlorethohydroxycarmalol (DPHC); Androgenic alopecia; Human dermal papilla cells; 5a-reductase; Wnt/(3-catenin signaling pathway; ISHIGE-OKAMURAE; FOLLICLE MORPHOGENESIS; BETA-CATENIN; ALOPECIA; PHLOROTANNINS; MECHANISMS; SKIN;

D O I：

10.1016/j.tiv.2025.106017

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

Diphlorethohydroxycarmalol (DPHC), a marine phlorotannin compound derived from the brown alga Ishige okamurae, has been known to have a variety of biological effects. Recently, marine resources have been highlighted by their effects on ameliorating alopecia and related hair loss. Therefore, this study aimed to investigate the potential of DPHC isolated from I. okamurae as a hair loss treatment through examination of its antiandrogenic alopecia effects. Molecular docking analysis predicted that DPHC can be used as a 5a-reductase inhibitor superior to finasteride, which has traditionally been used as an anti-androgenic alopecia agent. In addition, DPHC significantly inhibited 5a-reductase activity in dihydrotestosterone (DHT)-treated human dermal papilla (HDP) cells, and downregulated hair growth inhibitor proteins such as AR, DKK1, TGF-(31, and IL-6. Moreover, DPHC treatment remarkably upregulated both the phosphorylation levels of GSK3(3 and expression levels of (3-catenin in DHT-treated HDP cells, confirming the effects of DPHC on activating the Wnt/(3-catenin signaling pathway. Therefore, these findings suggest that DPHC has a significant potential to prevent androgenic alopecia by promoting hair growth and preventing hair loss.

引用

页数：10