Alleviating lanthanum stress in tomato plants using MnO nanoparticles and triacontanol: Impacts on growth, photosynthesis, and antioxidant defense

The present study evaluated the synthesis, characterization, and ameliorative potential of manganese oxide nanoparticles (MnO NPs) against lanthanum (La)-induced stress in tomato plants. Biosynthesized MnO NPs exhibited a characteristic UV-Vis absorption peak at 276 nm and a cubic crystalline structure with an average crystallite size of 13 nm, as determined by XRD. TEM images confirmed pseudo-spherical morphology and homogenous distribution. Under La stress, tomato plants showed a significant reduction in shoot length (54.90 %), root length (62.39 %), shoot dry weight (49.71 %), and root dry weight (37.17 %). Application of MnO NPs and triacontanol (TRIA) mitigated these effects, with combined treatments enhanced shoot and root lengths by 155.81 % and 216.66 %, respectively, and dry weights by 116.58 % (shoot) and 173.06 % (root). La stressed plants demonstrated decreased accumulation of La in roots and shoots by about 36.64 % and 32.21 %, respectively, upon synergistic application of MnO NPs and TRIA. La stress decreased photosynthetic pigments, including chlorophyll a (53.56 %), chlorophyll b (51.28 %), total chlorophyll (53.10 %), and carotenoids (26.36 %). Combined MnO NPs and TRIA treatment significantly increased these pigments by 110.23 %, 263.15 %, 142.27 %, and 266.66 %, respectively. Photosynthetic efficiency parameters, such as net photosynthetic rate, stomatal conductance, and transpiration rate, also improved by up to 74.44 %, 119.00 %, and 89.44 %, respectively, under combined treatments. Relative water content (RWC) decreased by 49.83 % under La stress but increased by 84.75 % following combined treatments. Osmolytes like proline and glycine betaine were elevated by 20.13 % and 38.47 %, respectively. Reactive oxygen species (ROS)-related markers, including H2O2, malondialdehyde, and electrolyte leakage, were significantly reduced by 58.14 %, 28.46 %, and 39.81 %, respectively, with MnO NPs and TRIA. Antioxidant enzyme activities were enhanced, with combined treatments elevating SOD (27.02 %), CAT (15.38 %), APX (90.37 %), and GR (90.38 %). Moreover, activities of DHAR and MDHAR, previously suppressed by La, increased by 91.64 % and 81.75 %, respectively. The findings highlight the synergistic role of MnO NPs and TRIA in alleviating La toxicity by enhancing growth, photosynthetic efficiency, antioxidant defense, and reducing ROS, offering a sustainable approach for crop improvement under metal stress conditions.