Assessing E. coli levels in surface soils of informal settlements using boot sock and standard grab methods

被引：0

作者：

Bata, Lamiya ^{[1
]}

Henry, Rebekah ^{[1
,2
]}

Barker, S. Fiona ^{[2
]}

Boyce, John ^{[3
]}

Lynch, Fiona ^{[2
]}

Vilsoni, Silvia Rosovoa ^{[4
]}

Tela, Autiko ^{[4
]}

Vamosi, Revoni ^{[4
]}

Taruc, Ruzka R. ^{[2
,5
]}

Agussalim, Andi Zulkifli ^{[5
]}

Saifuddaolah, Maghfira ^{[5
]}

Handis, Zainal ^{[5
]}

Mccarthy, David ^{[1
,6
,7
]}

Leder, Karin ^{[2
]}

RISE Consortium ^{[8
]}

机构：

[1] Monash Univ, Dept Civil Engn, Clayton, Vic 3800, Australia

[2] Monash Univ, Sch Publ Hlth & Prevent Med, Melbourne, Vic 3004, Australia

[3] Monash Univ, Biomed Discovery Inst, Ctr Impact AMR, Dept Microbiol,Infect Program, Clayton, Vic 3800, Australia

[4] Fiji Natl Univ, Sch Publ Hlth & Primary Care, Tamavua, Suva, Fiji

[5] Revitalising Informal Settlements & their Environm, Makassar, Indonesia

[6] Queensland Univ Technol, Sch Civil & Environm Engn, Brisbane, Qld 4000, Australia

[7] Univ Guelph, Sch Environm Sci, Guelph, ON, Canada

[8] Royal Melbourne Hosp, Victorian Infect Dis Serv, Melbourne, Vic 3052, Australia

来源：

SCIENCE ADVANCES | 2025年 / 11卷 / 11期

基金：

英国惠康基金; 英国医学研究理事会;

关键词：

MICROBIAL DIVERSITY; DIARRHEAL PATHOGENS; YOUNG-CHILDREN; HEALTH; INGESTION; WATER; CONTAMINATION; TRANSMISSION; SALMONELLA; SANITATION;

D O I：

10.1126/sciadv.adq9869

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Rapid urbanization leads to the growth of informal settlements, where inadequate sanitation infrastructure is common, thus promoting environmental contamination and risk of gastrointestinal infection. Soil contamination contributes to the transmission of enteropathogens, but traditional sampling approaches may poorly indicate public health risks due to limited spatial representation. This study compares traditional grab sampling of soil with a boot sock method, a composite technique designed to better reflect human-pathogen interactions. The boot sock method provided more sensitive detection of E. coli and lower inter-replicate variation compared to grab samples. Post hoc power analyses indicated that the boot sock technique required fewer samples to achieve adequate spatial representation across a sampling area than grab samples, potentially improving time and cost efficiency in pathogen exposure risk estimation.

引用

页数：8