Alleviative effects of green-fabricated zinc oxide nanoparticles on acrylamide-induced oxidative and inflammatory reactions in the rat stomach via modulating gastric neuroactive substances and the MiR-27a-5p/ROS/NF-κB axis

被引：1

作者：

Abd-Elhakim, Yasmina M. ^{[1
]}

Mohamed, Amany Abdel-Rahman ^{[1
]}

Khamis, Tarek ^{[2
,3
]}

Metwally, Mohamed M. M. ^{[4
,5
]}

El-Shetry, Eman S. ^{[6
,7
]}

Albaqami, Amirah ^{[8
]}

Mawkili, Wedad ^{[9
]}

Alosaimi, Manal E. ^{[10
]}

Alotaibi, Badriyah S. ^{[11
]}

Elashmouny, Naira ^{[12
]}

Dahran, Naief ^{[13
]}

Alsharif, Ghadi ^{[14
,15
]}

Samak, Mai A. ^{[16
]}

机构：

[1] Zagazig Univ, Fac Vet Med, Dept Forens Med & Toxicol, Zagazig 44519, Egypt

[2] Zagazig Univ, Fac Vet Med, Dept Pharmacol, Zagazig 44519, Egypt

[3] Zagazig Univ, Fac Vet Med, Lab Biotechnol, Zagazig 44519, Egypt

[4] King Salman Int Univ, Fac Vet Med, Dept Pathol & Clin Pathol, Ras Sidr, Egypt

[5] Zagazig Univ, Fac Vet Med, Dept Pathol, Zagazig 44519, Egypt

[6] Univ Hail, Coll Med, Dept Anat, Hail, Saudi Arabia

[7] Zagazig Univ, Fac Med, Dept Human Anat & Embryol, Zagazig 44519, Egypt

[8] Taif Univ, Turabah Univ Coll, Dept Clin Lab Sci, Taif 21944, Saudi Arabia

[9] Princess Nourah Bint Abdulrahman Univ, Coll Med, Dept Basic Sci, POB 84428, Riyadh 11671, Saudi Arabia

[10] Princess Nourah Bint Abdulrahman Univ, Coll Pharm, Dept Pharmaceut Sci, POB 84428, Riyadh 11671, Saudi Arabia

[11] Kafrelsheikh Univ, Dept Histol & cell Biol, Kafrelsheikh, Egypt

[12] Univ Jeddah, Dept Basic Med Sci, Jeddah, Saudi Arabia

[13] King Saud Bin Abdulaziz Univ Hlth Sci, Coll Appl Med Sci, Dept Clin Lab Sci, POB 9515, Jeddah 21423, Saudi Arabia

[14] King Abdullah Int Med Res Ctr, Dept Biomed Res, POB 9515, Jeddah 21423, Saudi Arabia

[15] Zagazig Univ, Fac Med, Dept Med Histol & Cell Biol, Zagazig 44519, Egypt

[16] Univ Hail, Coll Med, Hail 2240, Saudi Arabia

来源：

TISSUE & CELL | 2024年 / 91卷

关键词：

Acrylamide; Biosynthesized nanoparticles; Stomach; VACHT; EGFR; MiR-27a-5p; NF-KAPPA-B; NITRIC-OXIDE; MUCOSAL DEFENSE; TOXICITY; EXPOSURE; CELL; DEFICIENCY; APOPTOSIS;

D O I：

10.1016/j.tice.2024.102574

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

Little is known about the effects of acrylamide (AMD) on the stomach. So, this study evaluated the effect of oral AMD exposure (20 mg/kg b.wt) on oxidative status, apoptotic, and inflammatory reactions in rat's stomach for 60 days. To explore novel targets of AMD toxicity, a more detailed molecular and immune-expression study was performed. Besides, the possible protective effect of green synthesized zinc oxide nanoparticles (G-ZNP) (10 mg/ kg b.wt) was explored. The results revealed that AMD significantly provoked oxidative and lipid peroxidative damage of the stomach in terms of increased ROS and MDA but reduced SOD, CAT, GSH, and GSH/GSSG. Additionally, the stomachs of AMD-exposed rats showed a significant increment of PGE2 but reduced NO. Histopathologically, AMD induced a significant increase in PAS stain and the immunoexpression of iNOS and NF kappa B in the glandular stomach. A significant upregulation of CART, VACHT, EGFR, caspase-3, NOS-1, and miR-27a5p was evident in the stomach of the AMD group. Yet, G-ZNP oral dosing significantly rescued the AMD-induced oxidative damage, apoptotic reaction, inflammatory effect, and altered miR-27a-5p and gene expressions in the stomach. Conclusively, these findings demonstrated the efficacy of G-ZNP in protecting against the harmful impacts of acrylamide on stomach tissues.

引用

页数：13