Hierarchical Au@Pt nanoparticle/amino benzoic acid polymer-based hybrid material for labeled and label-free detection of interleukin-6: a comparative assessment

被引：0

作者：

Soto, Dayana ^{[1
,3
]}

Serafin, Veronica ^{[2
]}

Pedrero, Maria ^{[2
]}

Pingarron, Jose M. ^{[2
]}

Campuzano, Susana ^{[2
]}

Orozco, Jahir ^{[1
]}

机构：

[1] Univ Antioquia, Max Planck Tandem Grp Nanobioengieneering, Complejo Ruta N,Calle 67 52-20, Medellin 050010, Colombia

[2] Univ Complutense Madrid, Fac CC Quim, Dept Quim Analit, Pza Ciencias 2, Madrid 28040, Spain

[3] Cecoltec Serv SAS Co, Univ EAFIT, CECOLTEC Grp, Edificio Ingn, Bloque 19, Medellin 050022, Colombia

来源：

MICROCHIMICA ACTA | 2024年 / 191卷 / 11期

关键词：

Hybrid nanomaterial; Multi-walled carbon nanotubes; Electrochemical detection; Screen-printed carbon electrode; Differential pulse voltammetry; Gold nanoparticles; Label-free immunosensor; Sandwich-like immunosensor; IMMUNOSENSOR;

D O I：

10.1007/s00604-024-06745-y

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Interleukin-6 (IL6) is a cytokine mainly involved in inflammatory processes associated with various diseases, from rheumatoid arthritis and pathogen-caused infections to cancer, where malignant cells exhibit high proliferation and overexpression of cytokines, including IL6. Furthermore, IL6 plays a fundamental role in detecting and differentiating tumor cells, including colorectal cancer (CRC) cells. Therefore, given its range of biological activities and pathological role, IL6 determination has been claimed for the diagnosis/prognosis of immune-mediated diseases. Herein, a comparative study is presented of labeled and label-free electrochemical immunosensors involving a hierarchical Au@Pt nanoparticle/polymer hybrid material for detecting IL6. The electrochemical immunosensors were independently coupled to the surface of screen-printed carbon electrodes (SPCEs) previously modified with polymeric layers. While in the label-free immunosensor, an anti-IL6 antibody (IL6-Ab) was covalently bound to the modified SPCE surface, in the sandwich-like amperometric immunosensor, an anti-biotinylated-IL6 antibody (B-IL6-Ab) was attached to the electrode through biotin-avidin affinity interactions. The label-free format employed a straightforward detection of IL6 by differential pulse voltammetry (DPV). The resulting electrochemical immunosensors exhibited a linear dynamic range from 50 to 750 pg/mL IL6, with detection limits (LOD) of 14.4 and 6.0 pg/mL for label-free and sandwich-like immunosensors, respectively. This outstanding performance makes them versatile platforms for clinical analysis of a panel of biomarkers for early diagnosis/prognosis of inflammatory processes associated with oncological diseases, among other pathologies.

引用

页数：13