Detection of Ochratoxin A Using Immunoaffinity Chromatography Combined with Enzyme-Linked Immunosorbent Assay

被引：0
|
作者：
Zhang Y. [1 ]
Zhang X. [1 ]
Yuan Y. [1 ,2 ]
Zhang W. [1 ,2 ]
机构：
[1] College of Food Science and Technology, Hebei Agricultural University, Baoding
[2] Hebei Key Laboratory of Analysis and Control of Zoonotic Pathogenic Microorganism, Baoding
来源：
Shipin Kexue/Food Science | 2024年 / 45卷 / 10期
关键词：
detection; enzyme-linked immunosorbent assay; immunoaffinity chromatography; ochratoxin A;
D O I：
10.7506/spkx1002-6630-20231015-119
中图分类号：
学科分类号：
摘要：
Ochratoxin A (OTA) in samples was captured and concentrated by immunoaffinity chromatography (IAC) and detected by enzyme-linked immunosorbent assay (ELISA). The anti-OTA monoclonal antibodies used in IAC and ELISA came from different clone strains, belonging to different idiotypes, and there were differences between their selectivity for binding to OTA targets. The combined use of IAC and ELISA could effectively filter out the interference from OTA analogues, thus improving the specificity and sensitivity of immunological analysis. The limit of detection (LOD) for OTA in spiked samples was 0.2 ng/g, the quantification limit was 0.4 ng/g, and the average recovery was 75.9%. Although the recovery of the IAC-ELISA method was slightly lower than that of ELISA, the sensitivity was increased by 60 times. For 49 real samples, the proportion of samples that tested positive by this combined method was 100% consistent with the result of the national standard method, with a missed detection rate of 0%. Therefore, the developed method exhibited significant advantages in accuracy. As a comprehensive immunoassay, this method does not require any large-scale instruments or equipment, and has no strict requirement on the operating environment, thereby making it easy for grassroot laboratories to analyze OTA in samples. © 2024 Chinese Chamber of Commerce. All rights reserved.
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页码：257 / 264
页数：7
相关论文
共 18 条
  • [1] pp. 1-8, (2017)
  • [2] RANDA Z, ZAHOOR H U, NOOR A, Et al., Detection of multimycotoxins in camel feed and milk samples and their comparison with the levels in cow milk, Food Science & Nutrition, 10, 2, pp. 609-616, (2021)
  • [3] JAkUB T, VLADIMIR O, YANN G, Et al., Occurrence of ochratoxin A in Astragalus propinquus root and its transfer to decoction, Mycotoxin Research, 34, 3, pp. 223-227, (2018)
  • [4] HUANG X J, WANG S M, MAO D, Et al., Optimized QuEChERS method combined with UHPLC-MS/MS for the simultaneous determination of 15 mycotoxins in liquorice, Journal of AOAC International, 101, 3, pp. 633-642, (2018)
  • [5] ZHU Y, WANG S M, XU X H, Et al., Exposure assessment and risk-based limit levels evaluation of ochratoxin A in Astragali Radix in China, Ecotoxicology and Environmental Safety, 237, (2022)
  • [6] pp. 1-24, (2017)
  • [7] ZHANG Z W, HU X F, ZHANG Q, Et al., Determination for multiple mycotoxins in agricultural products using HPLC-MS/MS via a multiple antibody immunoaffinity column, Journal of Chromatography B, 1021, 5, pp. 145-152, (2016)
  • [8] CANPONE L, PICCINELLI A L, CELANO R, Et al., Rapid and automated on-line solid phase extraction HPLC-MS/MS with peak focusing for the determination of ochratoxin A in wine samples, Food Chemistry, 224, pp. 128-135, (2018)
  • [9] APPELL M, EVANS k O, JACkSON M A, Et al., Determination of ochratoxin A in grape juice and wine using nanosponge solid phase extraction clean up and liquid chromatography with fluorescence detection, Journal of Liquid Chromatography and Related Technologies, 41, pp. 949-954, (2018)
  • [10] pp. 37-43, (2009)
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