The Microstructure Analysis of Enzymes Immobilized by Sol-gel Method and Its Application in Phenol Optical Chemical Sensor

被引：0
|
作者：
Li, Jun [1 ]
Wang, Ke-Min [1 ]
Xiao, Dan [1 ]
Yang, Xiao-Hai [1 ]
机构：
[1] Coll. of Chem. and Chem. Engineering, Hunan University, Changsha 410082, China
来源：
| 2000年 / Higher Education Press卷 / 21期
关键词：
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The external states of horseradish peroxidase (HRP) encapsulated membrane prepared by enclosed aging method and dip-coating method are compared by common optical microscopy to clarify that the sol-gel membrane prepared by enclosed aging method is no cracking. The transmission electron microscopy (TEM) is used to observe the character of the sol-gel derived HRP membrane. The TEM photos show that enzyme can uniformly distribute in the gel as if in the solution and do not leach from the gel. This membrane can be used to fabricate a phenol optical sensor based on the decrease of chemiluminescence intensity. The response mechanism is discussed by compete reaction.
引用
收藏
相关论文
共 50 条
  • [1] The microstructure analysis of enzymes immobilized by sol-gel method and its application in phenol optical chemical sensor
    Li, J
    Wang, KM
    Xiao, D
    Yang, XH
    CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE, 2000, 21 (07): : 1018 - 1022
  • [2] Optical pH Sensor Based on Polyaniline Sol-Gel Film Immobilized with Bromothymol Blue and Phenol Red
    Othman, Norliza
    Hanim, Wan Fazlida
    Noor, Uzer Mohd
    Herman, Sukreen Hana
    INTERNATIONAL CONFERENCE ON ADVANCED SCIENCE, ENGINEERING AND TECHNOLOGY (ICASET 2015), 2016, 1774
  • [3] Application of the Sol-Gel Method at the Fabrication of Microstructure Fibers
    Jan Mrázek
    Vlastimil Matejec
    Ivan Kasik
    Milos Hayer
    Daniela Berková
    Journal of Sol-Gel Science and Technology, 2004, 31 : 175 - 178
  • [4] Application of the sol-gel method at the fabrication of microstructure fibers
    Mrázek, J
    Matejec, V
    Kasik, I
    Hayer, M
    Berková, D
    JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY, 2004, 31 (1-3) : 175 - 178
  • [5] The use of the sol-gel method for developing analytical devices based on immobilized enzymes
    Arsene, M
    Stanciu, L
    Parlog, C
    Bordeianu, L
    Raileanu, M
    REVUE ROUMAINE DE CHIMIE, 2002, 47 (12) : 1267 - 1271
  • [6] Phenol Biodegradation by Fungal Cells Immobilized in Sol-Gel Hybrids
    Tsekova, Kolishka V.
    Chernev, Georgi E.
    Hristov, Alexander E.
    Kabaivanova, Lyudmila V.
    ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES, 2013, 68 (1-2): : 53 - 59
  • [7] Preparation of immobilized glucose oxidase column by sol-gel technique and its application to the chemiluminescent glucose sensor
    Li, QW
    Wang, YM
    Zhang, XR
    Luo, GA
    CHINESE JOURNAL OF ANALYTICAL CHEMISTRY, 1999, 27 (11) : 1274 - 1277
  • [8] A comparison of immobilization sol-gel methods for an optical chemical hydrazine sensor
    Gojon, C
    Dureault, B
    Hovnanian, N
    Guizard, C
    SENSORS AND ACTUATORS B-CHEMICAL, 1997, 38 (1-3) : 154 - 162
  • [9] Production of Enzymes by Plant Cells Immobilized by Sol-Gel Silica
    Giovanna Pressi
    Roberto Dal Toso
    Renzo Dal Monte
    Giovanni Carturan
    Journal of Sol-Gel Science and Technology, 2003, 26 : 1189 - 1193
  • [10] Production of enzymes by plant cells immobilized by sol-gel silica
    Pressi, G
    Dal Toso, R
    Dal Monte, R
    Carturan, G
    JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY, 2003, 26 (1-3) : 1189 - 1193
12345