Optimization of the Reaction Conditions for the Peroxyoxalate Chemiluminescence Detection System of Fluorescent Compounds in a High-Performance Liquid Chromatography

被引：0
|
作者：
Faculty of Pharmaceutical Sciences, University of Tokyo, Hongo, Tokyo 113, Japan [1 ]
机构：
来源：
Anal. Sci. | / 5卷 / 713-719期
关键词：
Batch methods - Dansylated valine - Dipyridamole - Fluorescent compounds - Optimized conditions - Peroxyoxalate-chemiluminescence - Phosphate buffers - Reaction conditions;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Using an instrument of automatic fluid injection (batch method), we optimized such reaction conditions as pH, concentration and kinds of buffer for the peroxyoxalate chemiluminscence (PO-CL) detection of fluorescent compounds for high-performance liquid chromatography (HPLC). The results were compared with those obtained by HPLC, Consequently, the usefulness of the batch method for investigating the reaction conditions for PO-CL detection in HPLC was demonstrated. With the optimized condition using a phosphate buffer for the eluent in HPLC, the detection limits for Dns-Val and dipyridamole were 70 amol and 20 amol on column (S/N=2), respectively.
引用
收藏
相关论文
共 50 条
  • [1] Optimization of the reaction conditions for the peroxyoxalate chemiluminescence detection system of fluorescent compounds in a high-performance liquid chromatography
    Gohda, R
    Kimoto, K
    Santa, T
    Fukushima, T
    Homma, H
    Imai, K
    ANALYTICAL SCIENCES, 1996, 12 (05) : 713 - 719
  • [2] Optimization of the reaction conditions for the peroxyoxalate chemiluminescence detection system of fluorescent compounds in HPLC
    Imai, K
    Gohda, R
    Fukushima, T
    Santa, T
    Homma, H
    BIOMEDICAL CHROMATOGRAPHY, 1997, 11 (02) : 73 - 73
  • [3] POSTCOLUMN ADJUSTMENT OF CONDITIONS FOR PEROXYOXALATE CHEMILUMINESCENCE DETECTION FOR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
    HANAOKA, N
    JOURNAL OF CHROMATOGRAPHY, 1990, 503 (01): : 155 - 165
  • [4] Sensitive detection of near-infrared fluorescent dyes using high-performance liquid chromatography with peroxyoxalate chemiluminescence detection system
    Kimoto, K
    Gohda, R
    Murayama, K
    Santa, T
    Fukushima, T
    Homma, H
    Imai, K
    BIOMEDICAL CHROMATOGRAPHY, 1996, 10 (04) : 189 - 190
  • [5] STUDIES ON THE EFFECTS OF IMIDAZOLE ON THE PEROXYOXALATE CHEMILUMINESCENCE DETECTION SYSTEM FOR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
    IMAI, K
    NISHITANI, A
    TSUKAMOTO, Y
    WANG, WH
    KANDA, S
    HAYAKAWA, K
    MIYAZAKI, M
    BIOMEDICAL CHROMATOGRAPHY, 1990, 4 (03) : 100 - 104
  • [6] Determination of aromatic compounds by high-performance liquid chromatography with on-line photoreactor and peroxyoxalate chemiluminescence detection
    Ihara, Ayuko
    Kishikawa, Naoya
    Wada, Mitsuhiro
    Ohba, Yoshihito
    Nakashima, Kenichiro
    Kuroda, Naotaka
    LUMINESCENCE, 2007, 22 (06) : 567 - 574
  • [7] SENSITIVE ASSAY SYSTEM FOR NITROSAMINES UTILIZING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH PEROXYOXALATE CHEMILUMINESCENCE DETECTION
    FU, CG
    XU, HD
    WANG, Z
    JOURNAL OF CHROMATOGRAPHY, 1993, 634 (02): : 221 - 227
  • [8] Determination of nitrocatecholamines in rat brain using high-performance liquid chromatography-peroxyoxalate chemiluminescence reaction detection
    Tsunoda, Makoto
    Uchino, Erika
    Imai, Kazuhiro
    Hayakawa, Kazuichi
    Funatsu, Takashi
    JOURNAL OF CHROMATOGRAPHY A, 2007, 1164 (1-2) : 162 - 166
  • [9] DETERMINATION OF FEMTOMOLE CONCENTRATIONS OF CATECHOLAMINES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH PEROXYOXALATE CHEMILUMINESCENCE DETECTION
    HIGASHIDATE, S
    IMAI, K
    ANALYST, 1992, 117 (12) : 1863 - 1868
  • [10] CHEMILUMINESCENCE DETECTION SYSTEM FOR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
    IMAI, K
    METHODS IN ENZYMOLOGY, 1986, 133 : 435 - 449
12345