CHARACTERIZATION OF SACCHAROMYCES SP. THROUGH DETERMINATION OF AMINO ACID PROFILES BY CAPILLARY GAS CHROMATOGRAPHY.

The Saccharomyces strains uvarum, montuliensis, bayanus, capensis and florentinus were grown in liquid media prepared from a 1% aqueous glucose solution to which 0. 05% of ammonium sulfate or urea were added as the only nitrogen supply. The amino acids exuded during incubation were isolated by cation exchange and derivatized as the corresponding N,O-hepta-fluorobutyryl isopropyl esters. Separation was achieved on a highly deactivated glass capillary column coated with OV-101. A characteristic amino acid profile was obtained for each of the species studied, allowing the construction of a chemotaxonomic tree, on the basic of present or absent acids.