A method for the 32P labeling of peptides or peptide nucleic acid oligomers

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作者：
Salk Inst. for Biological Studies, P.O. Box 85800, San Diego, CA 92186, United States [1 ]
不详 [2 ]
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Bioconjugate Chem. | / 4卷 / 415-417期
关键词：
Nucleotides - Nucleic acids - Oligomers;
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摘要：
A novel approach to the radioactive labeling of peptides and PNA oligomers is described. It is based on the conjugation of a deoxynucleoside 3'-phosphate with the terminal amine of the substrate, followed by phosphorylation of the 5'-hydroxyl group of the nucleotide using T4 polynucleotide kinase and [γ-32P]ATP.
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