Membrane Associated RNA-Containing Vesicles Regulate Cortical Astrocytic Microdomain Calcium Transients in Awake Ischemic Stroke Mice

被引：0

作者：

Zhou, Zhongqiu ^{[1
]}

Bai, Ying ^{[1
]}

Gu, Xiaochun ^{[2
]}

Ren, Hui ^{[1
]}

Xi, Wen ^{[1
]}

Wang, Yu ^{[1
]}

Bian, Liang ^{[1
]}

Liu, Xue ^{[1
]}

Shen, Ling ^{[1
]}

Xiang, Xianyuan ^{[3
]}

Huang, Wenhui ^{[4
]}

Luo, Zhuojuan ^{[5
]}

Han, Bing ^{[1
]}

Yao, Honghong ^{[1
,6
,7
]}

机构：

[1] Southeast Univ, Sch Med, Dept Pharmacol, Jiangsu Prov Key Lab Crit Care Med, Nanjing 210009, Peoples R China

[2] Southeast Univ, Zhongda Hosp, Med Sch, Dept Radiol,Jiangsu Key Lab Mol & Funct Imaging, Nanjing 210009, Peoples R China

[3] Chinese Acad Sci, Shenzhen Inst Adv Technol, Shenzhen 518055, Peoples R China

[4] Univ Saarland, Ctr Integrat Physiol & Mol Med, Dept Mol Physiol, D-66421 Homburg, Germany

[5] Southeast Univ, Sch Life Sci & Technol, Key Lab Dev Genes & Human Dis, Nanjing 210096, Peoples R China

[6] Nantong Univ, Coinnovat Ctr Neuroregenerat, Nantong 226001, Peoples R China

[7] Southeast Univ, Inst Life Sci, Key Lab Dev Genes & Human Dis, Nanjing 210096, Peoples R China

来源：

ADVANCED SCIENCE | 2024年 / 11卷 / 46期

基金：

中国国家自然科学基金;

关键词：

astrocytic microdomain calcium transients; circSCMH1; ischemic stroke; membrane associated RNA-containing vesicles; mitochondria; PERIINFARCT DEPOLARIZATIONS; CA2+ TRANSIENTS; RELEASE; INJURY; PLASTICITY; CHANNELS;

D O I：

10.1002/advs.202404391

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Astrocytic processes minutely regulate neuronal activity via tripartite synaptic structures. The precision-tuning of the function of astrocytic processes is garnering increasing attention because of its significance in promoting brain repair following ischemic stroke. Microdomain calcium (Ca2+) transients in astrocytic processes are pivotal for the functional regulation of these processes. However, the understanding of the alterations and regulatory mechanism of microdomain Ca2+ transients during stroke remains limited. In the present study, a fast high-resolution, miniaturized two-photon microscopy is used to show that the levels of astrocytic microdomain Ca2+ transients are significantly reduced in the peri-infarct area of awake ischemic stroke mice. This finding correlated with the behavioral deficits shown by these mice under freely-moving conditions. Mitochondrial Ca2+ activity is an important factor driving the microdomain Ca2+ transients. DEAD Box 1 (DDX1) bound to circSCMH1 (a circular RNA involved in vascular post-stroke repair) facilitates the formation of membrane-associated RNA-containing vesicles (MARVs) and enhances the activity of astrocytic microdomain Ca2+ transients, thereby promoting behavioral recovery. These results show that targeting astrocytic microdomain Ca2+ transients is a potential therapeutic approach in stroke intervention.

引用

页数：18