Eco-friendly Ag NPs synthesis using an aqueous extract of Pistacia leaves as a new nanocatalyst for the A3 coupling reaction and investigation of its antioxidant and anti-glioma activities

In recent years, silver nanoparticles (AgNPs) have received a lot of attention due to their unique production characteristics. In the present research, we synthesized AgNPs/Pistacia by a green method using Pistacia extract, and various analytical characterization method including UV-Visible Spectroscopy, FT-IR, FE-SEM, EDX, TEM and XRD were applied to analyze the structure. Next, after determining the characteristics of AgNPs/Pistacia, we investigated its application as a reusable efficient nanocatalyst in A(3) coupling reaction for the synthesis of propargylamine derivatives. The desired products were obtained with good yields and the catalyst was isolated by centrifuge and reused for 8 consecutive times. The synthesized AgNPs/Pistacia were utilized in the investigation of cytotoxicity and the effects against various human glioma cells in vitro. Notably, the nano-drug demonstrated considerable resistance against the specified cell lines in a manner that was dependent on both time and concentration, as evaluated using the MTT. The yellow solution of MTT is converted into purple formazan crystals, which are subsequently measured spectrophotometrically at a wavelength of 570 nm. Notably, the percentage of cell viability in cancer cells decreased as the concentrations or doses of the nano-formulated drug increased. The AgNPs/Pistacia exhibited IC50 values of 94, 59 and 77 mu g/mL against the LN-229, U-87 and A-172 cells, respectively. The antioxidant properties of AgNPs/Pistacia were assessed using the DPPH assay. The current study illustrates the anti-glioma efficacies of AgNPs/Pistacia, suggesting their potential application as an anticancer supplement for the prevention and treatment of various cancers.