Determination of gatifloxacin in bulk and tablet preparations by high-performance liquid chromatography

被引：0
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作者：
Salgado, Hérida R. N. [1 ]
Lopes, Cristiani C. G. O. [1 ]
机构：
[1] Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas, UNESP, Rod. Araraquara-Jaú, km 1, CEP 14801-902, Araraquara, SP, Brazil
来源：
Journal of AOAC International | 2006年 / 89卷 / 03期
关键词：
A sensitive; precise; and specific high-performance liquid chromatographic (HPLC) method was developed for the assay of gatifloxacin (GATX) in raw material and tablets. The method validation parameters yielded good results and included the range; linearity; precision; accuracy; specificity; and recovery. It was also found that the excipients in the commercial tablet preparation did not interfere with the assay. The HPLC separation was carried out by reversed-phase chromatography on a C18 absorbosphere column (250 × 4.6 mm id; 5 μm particle size) with a mobile phase composed of acetic acid 5%-acetonitrile- methanol (70 + 15 + 15; v/v/v) pumped isocratically at a flow rate of 1.0 mL/min. The effluent was monitored at 287 nm. The calibration graph for GATX was linear from 4.0 to 14.0 μg/mL. The interday and intraday precisions (relative standard deviation) were less than 1.05%;
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Journal article (JA)
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页码：642 / 645
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