The Isolation and Purification of Muropeptides from the Bacillus subtilis and Its Effect on Spore Germination

被引：0
|
作者：
Liang D. [1 ,2 ]
Chen F. [1 ,2 ]
Zhang L. [1 ,2 ]
Hu X. [1 ,2 ]
机构：
[1] College of Food Science and Nutritional Engineering, China Agricultural University, Beijing
[2] National Engineering Research Center for Fruits and Vegetables Processing, China Agricultural University, Beijing
来源：
Journal of Chinese Institute of Food Science and Technology | 2021年 / 21卷 / 03期
关键词：
Muropeptides; Separation and purification; Spore germination; Structure identification;
D O I：
10.16429/j.1009-7848.2021.03.042
中图分类号：
学科分类号：
摘要：
The muropeptides was the fractions of peptidoglycan from cell wall after digested by mutanolysin. It could induce the spore germination. In this paper, the muropeptides were prepared from the cell wall of Bacillus. subtilis 168 by the method of enzymatic hydrolysis. Then, the high-performance liquid was used to separate and purified the muropeptides. In addition, the structure of muropeptides was identified by liquid chromatography-mass spectrometry, Fourier transform infrared spectroscopy and nuclear magnetic resonance, and the effect of muropeptides on spore germination was further studied. The results showed that the smallest unit of muropeptides obtained after separation and purification was disaccharide tripeptide, and it could induce about 90% spore to germinate. © 2021, Editorial Office of Journal of CIFST. All right reserved.
引用
收藏
页码：369 / 374
页数：5
相关论文
共 17 条
  • [1] ABDELMADJID A, GEROLD B, GUNTER A, Et al., Analysis of peptidoglycan structure from vegetative cells of Bacillus subtilis 168 and role of PBP 5 in peptidoglycan maturation, Journal of Bacteriology, 181, 13, pp. 3956-3966, (1999)
  • [2] REINEKE K, MATHYS A, HEINZ V, Et al., Mechanisms of endospore inactivation under high pressure, Trends in Microbiology, 21, 6, pp. 296-304, (2013)
  • [3] ATRIH A, ZOLLNER P, ALLMAIER G, Et al., Structural analysis of Bacillus subtilis 168 endospore peptidoglycan and its role during differentiation, Journal of Bacteriology, 178, 21, pp. 6173-6183, (1996)
  • [4] JONGE B L M D, CHANG Y S, GAGE D, Et al., Peptidoglycan composition of a highly methicillin-resistant Staphylococcus aureus strain. The role of penicillin binding protein 2A, The Journal of Biological Chemistry, 267, 16, pp. 11248-11254, (1992)
  • [5] YI X, SETLOW P., Studies of the commitment step in the germination of spores of Bacillus species, Journal of Bacteriology, 192, 13, pp. 3424-3433, (2010)
  • [6] STEPHEN E G, LETICIA A M C, AUDE A, Et al., Nod1 detects a unique muropeptide from gram-negative bacterial peptidoglycan, Science, 300, 5625, pp. 1584-1587, (2003)
  • [7] HAMM P, LIM M, HOCHSTRASSER R M., Structure of the amide I band of peptides measured by femtosecond nonlinear-infrared spectroscopy, The Journal of Physical Chemistry B, 102, 31, pp. 6123-6138, (1998)
  • [8] KACURAKOVA M P, CAPEKA V S, WELLNERB N, Et al., FT-IR study of plant cell wall model compounds pectic polysaccharides and hemicelluloses, Carbohydrate Polymers, 43, 2, pp. 195-203, (2000)
  • [9] LEE M, HESEK D, SHAH I M, Et al., Synthetic peptidoglycan motifs for germination of bacterial spores, ChemBio Chem, 11, 18, pp. 2525-2529, (2010)
  • [10] GIRARDIN S E, TRAVASSOS L H, HERVE M, Et al., Peptidoglycan molecular requirements allowing detection by Nod1 and Nod2, Journal of Biological Chemistry, 278, 43, pp. 41702-41708, (2003)
12