Evaluating Reproducibility and Similarity of Mass and Intensity Data in Complex Spectra-Applications to Tubulin

被引：0
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作者：
Olson, Matthew T. [1 ]
Blank, Paul S. [1 ]
Sackett, Dan L. [2 ]
Yergey, Alfred L. [1 ]
机构：
[1] Laboratory of Cellular and Molecular Biophysics, National Institute of Child Health and Human Development, NIH, Bethesda, MD, United States
[2] Laboratory of Integrative and Medical Biophysics, National Institute of Child Health and Human Development, NIH, Bethesda, MD, United States
来源：
Journal of the American Society for Mass Spectrometry | 2008年 / 19卷 / 03期
关键词：
We present a data processing approach based on the spectral dot product for evaluating spectral similarity and reproducibility. The method introduces 95% confidence intervals on the spectral dot product to evaluate the strength of spectral correlation; it is the only calculation described to date that accounts for both the non-normal sampling distribution of the dot product and the number of peaks the spectra have in common. These measures of spectral similarity allow for the recursive generation of a consensus spectrum; which incorporates the most consistent features from statistically similar replicate spectra. Taking the spectral dot product and 95% confidence intervals between consensus spectra from different samples yields the similarity between these samples. Applying the data analysis scheme to replicates of brain tubulin CNBr peptides enables a robust comparison of tubulin isotype expression and post-translational modification patterns in rat and cow brains. © 2008 American Society for Mass Spectrometry;
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页码：367 / 374
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