Identification of lactobacilli isolated from the cloaca and vagina of laying hens and characterization for potential use as probiotics to control Salmonella Enteritidis

被引：0
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作者：
Van Coillie, E. [1 ,4 ]
Goris, J. [1 ]
Cleenwerck, I. [2 ]
Grijspeerdt, K. [1 ]
Botteldoorn, N. [1 ]
Van Immerseel, F. [3 ]
De Buck, J. [3 ]
Vancanneyt, M. [2 ]
Swings, J. [2 ]
Herman, L. [1 ]
Heyndrickx, M. [1 ]
机构：
[1] Flemish Government, Institute for Agricultural and Fisheries Research, Unit Technology and Food, Melle, Belgium
[2] BCCM/LMG Bacteria Collection, Laboratory of Microbiology, Ghent University, Ghent, Belgium
[3] Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium
[4] Institute for Agricultural and Fisheries Research (ILVO), Unit Technology and Food, Brusselsesteenweg 370, B-9090 Melle, Belgium
来源：
Journal of Applied Microbiology | 2007年 / 102卷 / 04期
关键词：
Aims: To select Lactobacillus strains from laying hens for potential use as probiotic to control Salmonella Enteritidis infection. Methods and Results: One hundred and eighty-six lactobacilli were isolated from the cloaca and vagina of laying hens; and identified at the species level by a polyphasic taxonomic approach. All isolates belonged to the Lactobacillus acidophilus; Lactobacillus reuteri or Lactobacillus salivarius phylogenetic groups; with the L. reuteri group being the most predominant group. Based on genetic diversity; about 50 representative strains were selected and tested for in vitro properties that could be predictive for probiotic activity in laying hens. Salmonella inhibition was shown to be species dependent; and correlated to some extent with the production of lactic acid. A selection of strains was evaluated in a S. Enteritidis challenge experiment. Two strains; L. reuteri R-17485 and Lactobacillus johnsonii R-17504 significantly decreased the colonization of chicks by S. Enteritidis in caeca; liver and spleen. Conclusions: Lactobacilli isolated from laying hens were observed to inhibit Salmonella growth in vitro; most probably through production of lactic acid; and to decrease in vivo the S. Enteritidis colonization of chicks. Significance and Impact of the Study: The data demonstrate that Lactobacillus isolates from laying hens may have probiotic potential in reducing S. Enteritidis infection;
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页码：1095 / 1106
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