A disposable sensor based on immobilization of acetylcholinesterase to multiwall carbon nanotube modified screen-printed electrode for determination of carbaryl

被引：0
|
作者：
Cai, Jie [1 ]
Du, Dan [2 ]
机构：
[1] Technology Center of Wuhan Iron and Steel Company, Wuhan 430080, China
[2] Key Laboratory of Pesticide and Chemical Biology, Central China Normal University, Ministry of Education, Wuhan 430079, China
来源：
Journal of Applied Electrochemistry | 2008年 / 38卷 / 09期
关键词：
A simple method has been devised for immobilization of acetylcholinesterase (AChE) covalent bonding to a multiwall carbon nanotube (MWNT)-cross-linked cellulose acetate composite on a screen-printed carbon electrode (SPCE) and a sensitive and disposable amperometric sensor for rapid determination of carbaryl pesticide is proposed. The immobilized enzyme was preserved on this film because of the excellent biocompatibility and non-toxicity of cellulose acetate. Based on the inherent conductive properties of the MWNT; the immobilized AChE had greater affinity for ATCl and excellent catalytic effect in the hydrolysis of ATCl. MWNT improved the interface enzymatic hydrolysis reaction and increased the amperometric response of the sensor. Under optimum conditions; the inhibition of carbaryl on AChE increased linearly with the increasing concentration of carbaryl in two ranges; from 0.01 to 0.5 μg mL-1 and from 2 to 20 μg mL-1; with the correlation coefficients of 0.9985 and 0.9977; respectively. The detection limit was 0.004 μg mL -1 taken as the concentration equivalent to 10% decrease in signal. The sensor showed acceptable stability; accuracy and could be fabricated in batches; thus it is economic and portable. This type of disposable enzyme-based amperometric sensor has extensive application potential in environmental monitoring of pesticides;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Journal article (JA)
引用
收藏
页码：1217 / 1222
相关论文
共 50 条
  • [1] A disposable sensor based on immobilization of acetylcholinesterase to multiwall carbon nanotube modified screen-printed electrode for determination of carbaryl
    Jie Cai
    Dan Du
    Journal of Applied Electrochemistry, 2008, 38 : 1217 - 1222
  • [2] A disposable sensor based on immobilization of acetylcholinesterase to multiwall carbon nanotube modified screen-printed electrode for determination of carbaryl
    Cai, Jie
    Du, Dan
    JOURNAL OF APPLIED ELECTROCHEMISTRY, 2008, 38 (09) : 1217 - 1222
  • [3] Acetylcholinesterase sensor based on screen-printed carbon electrode modified with prussian blue
    Suprun, E
    Evtugyn, G
    Budnikov, H
    Ricci, F
    Moscone, D
    Palleschi, G
    ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2005, 383 (04) : 597 - 604
  • [4] Acetylcholinesterase sensor based on screen-printed carbon electrode modified with prussian blue
    E. Suprun
    G. Evtugyn
    H. Budnikov
    F. Ricci
    D. Moscone
    G. Palleschi
    Analytical and Bioanalytical Chemistry, 2005, 383 : 597 - 604
  • [5] A Disposable, Screen-Printed Electrode for the Amperometric Determination of Azide Based on the Immobilization with Catalase or Tyrosinase
    Yue Cui
    John P. Barford
    Reinhard Renneberg
    Analytical Sciences, 2006, 22 : 1279 - 1281
  • [6] A disposable, screen-printed electrode for the amperometric determination of azide based on the immobilization with catalase or tyrosinase
    Cui, Yue
    Barford, John P.
    Renneberg, Reinhard
    ANALYTICAL SCIENCES, 2006, 22 (10) : 1279 - 1281
  • [7] Nafion mixed carbon nanotube modified screen-printed carbon electrode as a disposable electrochemical sensor for quantification of Amitraz in honey and longan samples
    Lerdsri, Jamras
    Upan, Jantima
    Jakmunee, Jaroon
    ELECTROCHIMICA ACTA, 2022, 410
  • [8] Electrochemical Determination of Hydrazine Based on Modified Screen-Printed Carbon Electrode
    Mohammadi, Sayed Zia
    Tajik, Somayeh
    Hamidian, Hooshang
    Mousazadeh, Farideh
    Emambakhsh, Fatemeh
    Sajadi, Pari-Sima
    Allahabadi, Hossein
    JOURNAL OF THE ELECTROCHEMICAL SOCIETY, 2025, 172 (01)
  • [9] A disposable molecularly imprinted electrochemical sensor based on screen-printed electrode modified with ordered mesoporous carbon and gold nanoparticles for determination of ractopamine
    Ma, Min
    Zhu, Pei
    Pi, Fuwei
    Ji, Jian
    Sun, Xiulan
    JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 2016, 775 : 171 - 178
  • [10] Amperometric fructose sensor based on ferrocyanide modified screen-printed carbon electrode
    Biscay, Julien
    Costa Rama, Estefania
    Gonzalez Garcia, Maria Begona
    Julio Reviejo, A.
    Pingarron Carrazon, Jose Manuel
    Costa Garcia, Agustin
    TALANTA, 2012, 88 : 432 - 438
12345