Alleviated cadmium toxicity in wheat (Triticum aestivum L.) by the coactive role of zinc oxide nanoparticles and plant growth promoting rhizobacteria on TaEIL1 gene expression, biochemical and physiological changes

Cadmium (Cd) contamination in agricultural soil is a major global concern among the multitude of human health and food security. Zinc oxide nanoparticles (ZnO-NPs) and plant growth promoting rhizobacteria (PGPR) have been known to combat heavy metal toxicity in crops. Herein, the study intended to explore the interactive effect of treatments mediated by inoculation of PGPR and foliar applied ZnO-NPs to alleviate Cd induced phytotoxicity in wheat plants which is rarely investigated. For this purpose, TaEIL1 expression, morpho-physiological, and biochemical traits of wheat were examined. Our results revealed that Cd reduced growth and biomass, disrupted plant physiological and biochemical traits, and further expression patterns of TaEIL1. The foliar application of ZnO-NPs improved growth attributes, photosynthetic pigments, and gas exchange parameters in a dose-additive manner, and this effect was further amplified with a combination of PGPR. The combined application of ZnO-NPs (100 mg L-1) with PGPR considerably increased the catalase (CAT; 52.4%), peroxidase (POD; 57.4%), superoxide dismutase (SOD; 60.1%), ascorbate peroxidase (APX; 47.4%), leading to decreased malondialdehyde (MDA; 47.4%), hydrogen peroxide (H2O2; 38.2%) and electrolyte leakage (EL; 47.3%) under high Cd (20 mg kg-1) stress. Furthermore, results revealed a significant reduction in roots (56.3%), shoots (49.4%), and grains (59.4%) Cd concentration after the Combined treatment of ZnO-NPs and PGPR as compared to the control. Relative expression of TaEIL1 (two homologues) was evaluated under control (Cd 0), Cd, ZnO-NPs, PGPR, and combined treatments. Expression profiling revealed a differential expression pattern of TaEIL1 under different treatments. The expression pattern of TaEIL1 genes was upregulated under Cd stress but downregulated under combined ZnO-NPs and PGPR, revealing its crucial role in Cd stress tolerance. Inferentially, ZnO-NPs and PGPR showed significant potential to alleviate Cd toxicity in wheat by modulating the antioxidant defense system and TaEIL1 expression. By inhibiting Cd uptake, and facilitating their detoxification, this innovative approach ensures food safety and security. © 2024