Shatianyu (Citrus grandis L. Osbeck) whole fruit alleviated loperamide-induced constipation via enhancing gut microbiota-mediated intestinal serotonin secretion and mucosal barrier homeostasis

被引：0

作者：

Deng, Mei ^{[1
]}

Ye, Jiamin ^{[1
]}

Zhang, Ruifen ^{[1
]}

Zhang, Shuai ^{[1
]}

Dong, Lihong ^{[1
]}

Su, Dongxiao ^{[2
]}

Zhang, Mingwei ^{[1
]}

Huang, Fei ^{[1
]}

机构：

[1] Guangdong Acad Agr Sci, Sericultural & Agrifood Res Inst, Key Lab Funct Foods, Minist Agr,Guangdong Key Lab Agr Prod Proc, Guangzhou 510610, Peoples R China

[2] Guangzhou Univ, Sch Chem & Chem Engn, Guangzhou 510006, Peoples R China

来源：

FOOD & FUNCTION | 2024年 / 15卷 / 21期

基金：

中国国家自然科学基金;

关键词：

POLYSACCHARIDES; TRANSIT; PATHWAY; DIET;

D O I：

10.1039/d4fo02765e

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This study aims to explore the effects of Shatianyu (Citrus grandis L. Osbeck) whole fruit powder (SWFP) enriched in flavonoids and dietary fiber on loperamide-induced constipation after a 4-week administration in the diet, together with possible microbiota-mediated mechanisms. The SWFP intervention shortened the first defecation time and increased defecation frequency; it also increased the serum serotonin (5-HT) level and decreased the LPS level in constipation mice. SWFP promoted the development of colonic enterochromaffin cells (ECs) and upregulated the expression of 5-HT synthetic rate-limiting enzyme (Tph1) in ECs. Furthermore, SWFP downregulated the expression of colonic TLR-4, TNF-alpha and IL-1 beta and upregulated the expression of tight junction proteins. Besides promoting 5-HT secretion in ECs, butyrate was proved to play a positive role in enhancing intestinal barrier homeostasis through FFAR2/3. Notably, SWFP increased both the fecal butyrate contents and colonic FFAR3 expression in a dose-related manner. Likewise, SWFP enriched butyrate-production related microbes, such as Ruminococcus_torques_group, Ruminococcus, Dubosiella and Parasutterella. Thus, SWFP might alleviate constipation by regulating the microbiota to produce butyrate, thereby enhancing colonic 5-HT secretion and the FFAR3-mediated anti-inflammatory pathway.

引用

页码：10614 / 10627

页数：15