Biosynthesis of chiral epichlorohydrin by halohydrin dehalogenase based on Pickering emulsion system

被引：0
|
作者：
Tang X. [1 ]
Wang J. [1 ]
Zhu X. [1 ]
Zheng R. [1 ]
机构：
[1] College of Bioengineering, Zhejiang University of Technology, Zhejiang, Hangzhou
来源：
Huagong Xuebao/CIESC Journal | 2023年 / 74卷 / 07期
关键词：
biocatalysis; chiral epichlorohydrin; enzyme; Pickering emulsion; system optimization;
D O I：
10.11949/0438-1157.20230450
中图分类号：
学科分类号：
摘要：
Biosynthesis of chiral epichlorohydrin (ECH) from 1, 3-dichloro-2-propanol (1, 3-DCP) catalyzed by halohydrin dehalogenases is an efficient and green synthetic route which exhibits advantages of mild reaction conditions, high atomic economy and environmental friendliness. Therefore, it has broad application prospects. Since the substrate and product in this route is easily to be degraded in aqueous phase, a two-phase system of water and organic solvents was applied. In order to improve the stability of the enzyme in organic solvents, a Pickering emulsion system was constructed with modified SiO2 as emulsifier for the enzyme-medicated biocatalysis of chiral ECH, and its catalytic performance was characterized. The results showed that the stability of the Pickering emulsion-based halohydrin dehalogenase in the water-organic solvent two-phase system was significantly improved, and its half-life was increased from 9.7 h to 20.1 h. After that, the reaction conditions were optimized and 50 mmol/L 1,3-DCP was effectively converted within 40 min. The yield of product (S)-ECH reached 93.6% with e.e. value of 95.3%. The obtained results laid the foundation for highly efficient biocatalytic synthesis of chiral ECH. © 2023 Chemical Industry Press. All rights reserved.
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页码：2926 / 2934
页数：8
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