Immobilization of carbonic anhydrase on modified polyethylene membrane and silica

被引：0
|
作者：
Meng L. [1 ]
Chong R. [1 ]
Sun F. [1 ]
Meng Z. [1 ]
Liu W. [1 ]
机构：
[1] School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing
来源：
Huagong Xuebao/CIESC Journal | 2023年 / 74卷 / 08期
关键词：
carbonic anhydrase; enzyme activity; immobilization; polyethylene; silica; stability;
D O I：
10.11949/0438-1157.20230348
中图分类号：
学科分类号：
摘要：
The activity of carbonic anhydrase (CA) immobilized by different methods was investigated using modified polyethylene (PE) membrane and silica (SiO2) as carriers, and then modified with polydopamine/ polyethyleneimine (PDA/PEI) PE- and SiO2-immobilized CA were used as research objects. The optimum reaction conditions and stability were investigated. The results show that under the same reaction conditions, the activity recovery of PDA/PEI-SiO2 immobilized CA was the highest, which was 58.8%, and the activity recovery of PDA/ PEI-PE immobilized CA was 17.1%. Their retentive activities were 84.8% and 90.2%, respectively, after 10 use recycles. The optimum reaction conditions of immobilized enzymes were 35℃ and pH 8.5, which were the same as those of free enzymes. The stability of the two immobilized enzymes at higher temperature (55—65℃) and higher acid concentration (>0.010 mol/L) was better than that of free enzyme. Mg2+ could significantly promote the activity of free enzyme and immobilized enzyme, while K+ and Mg2+ had no obvious effect. PDA/PEI-SiO2 and PDA/PEI-PE immobilized CA retained 95.2% 和 92.4% activity after stored at 4℃ for 10 d. When used in the CO2 hydration reaction, the amount of CaCO3 produced was 120% and 70% of free CA. The immobilized CA has great potential in the industrial application of CO2 capture. © 2023 Materials China. All rights reserved.
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页码：3472 / 3484
页数：12
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