Enhancing the volatile organic compound and biomass production by three biocontrol potential bacteria in corn steep liquor growth medium and development of cell freeze-drying process

被引：0

作者：

Villanova, Valeria ^{[1
,2
]}

Andreolli, Marco ^{[2
,3
]}

Lampis, Silvia ^{[2
,3
]}

Panighel, Annarita ^{[4
]}

Flamini, Riccardo ^{[4
]}

Forte, Vally ^{[4
]}

Zapparoli, Giacomo ^{[2
]}

机构：

[1] Univ Palermo, Dept Biol Chem & Pharmaceut Sci & Technol STEBICEF, Viale Sci Edificio 16, I-90128 Palermo, Italy

[2] Univ Verona, Dept Biotechnol, Str Le Grazie 15, I-37134 Verona, Italy

[3] Univ Verona, VUCC DBT Verona Univ Culture Collect, Dept Biotechnol, Str Le Grazie 15, I-37134 Verona, Italy

[4] Res Ctr Viticulture & Enol CREA VE, Council Agr Res & Econ, Viale XXVIII Aprile 26, I-31015 Conegliano, TV, Italy

来源：

JOURNAL OF APPLIED MICROBIOLOGY | 2024年 / 135卷 / 11期

关键词：

antagonistic bacteria; Bacillus sp; corn steep liquor; PGP bacteria; Pseudomonas protegens; volatile organic compounds; COMPLEX; GRAPES;

D O I：

10.1093/jambio/lxae270

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aims: This study investigates the traits of three plant growth-promoting (PGP) and antagonistic bacteria, Pseudomonas protegens MP12, Bacillus sp. 3R4, and Bacillus sp. T22, to assess their potential application as biocontrol agents by using the ecofriendly and low-cost substrate Corn Steep Liquor (CSL) medium. Analyses of antagonism through volatile organic compounds (VOCs) production, biofilm formation, and growth performance were carried out. Methods and results: Dual antagonism assay showed that all strains displayed significant antagonistic activity against Botrytis cinerea through VOCs. Gas chromatography demonstrated that strains in the CSL exhibited higher VOCs production than nutrient medium. Moreover, enhanced biofilm formation analysed by Calgary Biofilm Device, growth, and biomass were noted in CSL cultures. Pseudomonas protegens MP12, which showed higher cell concentration and biomass yield, was selected for freeze-drying treatments. Storage cell viability assays evidenced that it can be effectively preserved for nearly 7 months at 4 degrees C. Conclusion: The results here obtained showed that CLS medium enhanced VOCs production, biofilm formation, growth, and biomass of the antagonistic bacteria of the three strains. Eventually, the more effective strain P. protegens MP12 can be stored for nearly 7 months at 4 degrees C.

引用

页数：9