Organic contaminants of emerging concern (OCECs) as a new class of organic pollutants have initiated intense global concerns. The efficient enzymatic water treatment was consider the crucial for the potential remediation process. However, the widespread application of enzymatic systems faces significant obstacles, including low enzyme activity and the lower value of recovery. Thus, a novel multi-enzymes immobilized biocatalytic system (multi-enzyme@COF@Chitosan aerogels) was synthesized via the combine the in-situ direct immobilization process and double-cross linking strategy in this study. Results showed that multi-enzyme@COF@Chitosan aerogels exhibits good mesoporous structure (the pore size distribution is 2.5 nm) and mechanical strength, leading to remarkable stability a broader pH range (4-7) and temperature range (30-70 degrees C). For typical OCECs, including 2-mercaptobenzothiazole (MBT), paracetamol (PCM), sulfamethoxazole (SMO), bisphenol A (BPA), Sulfamerazine (SMR), the remediation activities reached 100 %, 87 %, 68 %, 69 %, and 100 %, respectively. Likewise, the multi-enzyme@COF@Chitosan aerogels exhibited good reusability, with the relative activity still maintained approximately 75.7 % after seven cycles. Moreover, the electrochemical characteristics of multienzymes@COF@Chitosan aerogel and enzymes activities were contributed to degradation the OCECs.
