Mixotrophic Denitrification of Actual Tail Water from Wastewater Treatment Plant Treated by PHBV-Pyrite Based Constructed Wetlands

被引：0

作者：

Xu Z. ^{[1
]}

Zhou Q. ^{[2
]}

Jia L. ^{[2
]}

Wu W. ^{[2
]}

Xing C. ^{[1
]}

机构：

[1] School of Ecology and Environment, Zhengzhou University, Zhengzhou

[2] College of Environmental Sciences and Engineering, Peking University, Beijing

来源：

Beijing Daxue Xuebao (Ziran Kexue Ban)/Acta Scientiarum Naturalium Universitatis Pekinensis | 2024年 / 60卷 / 02期

关键词：

constructed wetland; mixotrophic denitrification; pyrite; solid carbon source; tail water;

D O I：

10.13209/j.0479-8023.2024.006

中图分类号：

学科分类号：

摘要：

Taking the tail water of a large wastewater treatment plant in Xinxiang city, Henan Province of China, as the research object, the deep nitrogen and phosphorus removal by constructed wetlands (CWs) for this tail water was studied. Polyhydroxybutyrate valerate (PHBV) and pyrite were selected as the main functional fillers of wetland system to construct a mixotrophic denitrification system by combining heterotrophic denitrification and autotrophic denitrification. Water quality index monitoring and high-throughput sequencing technology were used to explore the removal performance and microbial community structure. The PHBV+pyrite experimental group and PHBV+ ceramite control group were set up for continuous operation for 77 days. Results show that when hydraulic retention time (HRT) was 2 hours, NO3−-N, TN, TP and COD concentration are 8.39±1.72 mg/L, 10.41±1.58 mg/L, 0.37 mg/L and 15.7 mg/L, respectively, the average effluent TN concentration reaches at 2.22 mg/L, TP concentration reaches 0.28 mg/L and COD concentration is 32.7 mg/L. The nitrogen removal efficiency of CWs in PHBV+ pyrite group (78.55%) is obviously better than that in PHBV+ceramite group (51.25%), basically meeting the surface water environmental quality standard (GB 3838-2002) Class V. Analysis of the microbial community structure shows that the PHBV + pyrite group advantage bacterium in artificial wetland are autotrophic bacteria Desulfobacter and heterotrophic bacteria unclassified_k__norank_d__Bacteria, the abundance are 23.98% and 12.30% respectively. Mixotrophic denitrification is achieved through coordination of autotrophic and heterotrophic bacteria, in which autotrophic denitrification led by Desulfobacter related to sulfur metabolism may play a major role in systematic nitrogen removal. These results can provide reference for the engineering practice of biological nitrogen and phosphorus removal in tail water of PHBV+pyrite-based CWs. © 2024 Peking University. All rights reserved.

引用

页码：315 / 328

页数：13

共 28 条

[1] Zhou Q, Sun H, Jia L, Et al., Simultaneous biological removal of nitrogen and phosphorus from secondary effluent of wastewater treatment plants by advanced treatment: a review, Chemosphere, 296, (2022)
[2] 20, pp. 413-420
[3] Yang Z, Zhou Q, Sun H, Et al., Metagenomic analyses of microbial structure and metabolic pathway in solid-phase denitrification systems for advanced nitrogen removal of wastewater treatment plant effluent: a pilot-scale study, Water Research, 196, (2021)
[4] 38, 5, (2017)
[5] (2021)
[6] Li M, Duan R, Hao W, Et al., High-rate nitrogen removal from carbon limited wastewater using sulfur-based constructed wetland: impact of sulfur sources, Science of The Total Environment, 744, (2020)
[7] Chen Y, Wen Y, Zhou Q, Et al., Effects of plant biomass on nitrogen transformation in subsurface-batch constructed wetlands: a stable isotope and mass balance assessment, Water Research, 63, pp. 158-167, (2014)
[8] Zhang W, Huang F, Hu W., Performance and mechanism of synchronous nitrate and phosphorus removal in constructed pyrite-based mixotrophic denitrification system from secondary effluent, Environmental Science and Pollution Research International, 27, 29, pp. 36816-36825, (2020)
[9] Zhou Q, Jia L, Zhao L, Et al., Difference and network analysis of functional genes revealed the hot area of carbon degradation, nitrogen, phosphorus, and sulfur cycling in blending systems with pyrite and poly(3hydroxybutyrate-hydroxyvalerate) for nitrogen and phosphorus removal, ACS ES&T Water, 2, 6, pp. 1087-1098, (2022)
[10] Zhou Q, Sun H, Jia L, Et al., Simultaneously advanced removal of nitrogen and phosphorus in a biofilter packed with ZVI/PHBV/sawdust composite: deciphering the succession of dominant bacteria and keystone species, Bioresource Technology, 347, (2022)

← 1 2 3 →