High-throughput Analysis of Tyrosinase Activity and Inhibitors Based on Matrix-assisted Laser Desorption/ionization Mass Spectrometry

The efficient and convenient separation capability and strong ultraviolet absorption of magnetic graphene nanomaterials(Fe3O4@G) were applied in the analysis of tyrosinase activity and inhibitors using matrix-assisted laser desorption/ionization mass spectrometry(MALDI-MS). Compared to other methods, this approach reduces sample loss and improves detection sensitivity by avoiding cumbersome sample pretreatment processes. Additionally, the combination of MALDI-MS enables high-throughput sample analysis. The development of this research is of significant importance for gaining a deeper understanding of melanin synthesis processes, studying related diseases, and developing effective drugs and cosmetic products.