Analysis of Induced Resistant Volatile Compounds in Pinus yunnanensis var. tenuifolia Damaged by Tomicus minor (Coleoptera: Scolytidae) by HS-SPME-GC/MS Method

被引：0
|
作者：
Wu D. [1 ]
Jia J. [1 ]
Chen H. [1 ]
Yan P. [1 ]
Xu R. [2 ]
Yang L. [2 ]
Yang Z. [1 ]
机构：
[1] Guangxi Zhuang Autonomous Region Forestry Research Institute, Guangxi Masson Pine Engineering Technology Research Center, Nanning
[2] Guangxi State Owned Yachang Forest Farm, Leye
来源：
Linye Kexue/Scientia Silvae Sinicae | 2021年 / 57卷 / 06期
关键词：
Discriminant analysis; GC/MS; HS-SPME; Pinus yunnanensis; Tomicus minor; Volatile chemicals;
D O I：
10.11707/j.1001-7488.20210611
中图分类号：
学科分类号：
摘要：
【Objective】This study aims to understand the harmfulness of bark beetle,Tomicus minor,and the stress response of Pinus yunnanensis var. tenuifolia, and explore the relationship between the response and metabolic volatiles related to resistance so as to put forward effective control measures.【Method】The induced volatile substances of the pine were studied by headspace solid phase microextraction-gas chromatography/mass spectrometry with the shoots in different damage degrees in Yachang Forest Farm, Leye County, Guangxi. The optimum conditions of SPME were as follows: equilibrium for 15 min on solid phase extraction instrument at 75 ℃ and extraction for 15 min with 75 micron extraction head (DVB/CAR/PDMS).【Result】The most volatile components were detected in severely damaged shoots, mainly terpenes and alcohols. The main metabolic volatile products of the pine were closely related to its damage degree. With the increase of the damage degree, the contents of α-pinene, 3-pinene, β-caryophyllene and β-picengene increased, while the content of β-pinene decreased gradually, and only a small amount of longifolene was found in the control. In severely damaged shoots, β-pinene content was the lowest, only 47.85% of the control, while 3-pinene increased the most,higher than 62.83% of the control. The total amount of monoterpenoids and sesquiterpenoids oxygen-containing derivatives increased with the degree of damage, indicating that the insect resistance decreased with the increase of oxygen-containing compounds.【Conclusion】The differences in the contents of 3-carene, β-pinene, β-elemene, β-caryophyllene and β-bichenylsolanee are the main reasons for inducing resistance of the pine to the bark beetle in transverse pits.Headspace solid phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS) is suitable for the rapid analysis of volatile components in the pine needles, and this study provides a scientific basis for the study of induced resistance substances of Tomicus minor. © 2021, Editorial Department of Scientia Silvae Sinicae. All right reserved.
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页码：103 / 110
页数：7
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