Protective Effects of Maca (Lepidium meyenii Walp) Triterpenoid Fractions on Oxidative Damage of Nerve Cells Induced by H2O2

被引：0

作者：

Tang W. ^{[1
]}

Xie L. ^{[1
]}

Wang N. ^{[1
]}

Wang R. ^{[1
,2
]}

Jin L. ^{[1
]}

Chu B. ^{[1
]}

Zhang Y. ^{[1
]}

机构：

[1] College of Biosystems Engineering and Food Science, Zhejiang University, Zhejiang Engineering Center for Food Technology and Equipment, Hangzhou

[2] Department of Pharmacology and Key Lab of Ministry of Education in Fertility Preservation and Maintenance, Ningxia Medical University, Yinchuan

来源：

Zhang, Ying (yzhang@zju.edu.cn) | 2018年 / Chinese Institute of Food Science and Technology卷 / 18期

关键词：

Maca (Lepidium meyenii Walp); Nerve cell; Oxidative damage; Protective effects; Triterpenoid saponin;

D O I：

10.16429/j.1009-7848.2018.07.004

中图分类号：

学科分类号：

摘要：

Objective: The protective effects of triterpenoid saponin fractions on hydrogen peroxide induced damage in PC12 cell were studied. Methods: Crude Maca (Lepidium meyenii Walp) saponin was isolated with ultrasonic-assisted extraction technique and liquid-liquid extraction, which was further fractioned by D101 macroporous resin to obtain two fractions (F1 and F2). The total saponin, total flavone and total phenolic contents were comparatively analyzed by colorimetric method. And the ginsenoside saponin contents of two fractions were determined by UPLC-PDA. Furthermore, the protective effects of saponin fractions to oxidative damage in PC12 cell were assessed by MTT. Results: It revealed that F1 mainly contained saponin (ginsenoside saponin 19.5 mg/g), phenolic compounds and flavone; however, F2 contained much more saponin with ginsenoside saponin (270 mg/g). Both fractions played anti-oxidative activity in vitro and the MTT assay suggested that F2 (EC50=0.213 μg/mL) played stronger bioactivity than F1 (EC50=0.995 μg/mL). Taken together, the total saponin of maca was rich in triterpenoid saponin (mainly ginsenoside) and flavone which was benefit to prevent the oxidative damage in PC12 cell. It indicated that maca saponin could be explored as a potential function factor used to prevent neurodegenerative diseases in term of functional food or specific medical purpose. © 2018, Editorial Office of Journal of CIFST. All right reserved.

引用

页码：28 / 36

页数：8

共 17 条

[1] Zhang C., Hang L., Yao T., Et al., Parkin regulation and neurodegenerative disorders, Frontiers in Aging Neuroscience, 7, pp. 1663-4365, (2016)
[2] Jodeiri F.M., Ghaedi K., Megraw T.L., Et al., Does PGC1α/FNDC5/BDNF elicit the beneficial effects of exercise on neurodegenerative disorders?, Neuro Molecular Medicine, 18, 1, pp. 1-15, (2016)
[3] An J.M., Moon S.A., Hong S.Y., Et al., Neuroprotective effect of 3-morpholinosydnonimine against Zn<sup>2+</sup>-induced PC12 cell death, European Journal of Pharmacology, 748, pp. 37-44, (2015)
[4] Deng Q., Yang X., Protective effects of Gynostemma pentaphyllum polysaccharides on PC12 cells impaired by MPP(+), International Journal of Biological Macromolecules, 69, 8, pp. 171-175, (2014)
[5] Zhang H., Cao Y., Chen L., Et al., A polysaccharide from Polygonatum sibiricum attenuates amyloid-β-induced neurotoxicity in PC12 cells, Carbohydrate Polymers, 117, pp. 879-886, (2015)
[6] Massoma L.D., Gasco M., Gonzales G.F., Fertility and estrogenic activity of Turraeanthus africanus in combination with Lepidium meyenii (Black maca) in female mice, European Journal of Integrative Medicine, 4, 3, pp. 345-351, (2012)
[7] Gonzales G.F., Cordova A., Vega K., Et al., Effect of Lepidium meyenii (Maca), a root with aphrodisiac and fertility-enhancing properties, on serum reproductive hormone levels in adult healthy men, Journal of Endocrinology, 176, 1, pp. 163-168, (2003)
[8] Gonzales G.F., Gasco M., Lozada-Requena I., Role of maca (Lepidium meyenii) consumption on serum interleukin-6 levels and health status in populations living in the peruvian central andes over 4000 m of altitude, Plant Foods for Human Nutrition, 68, 4, pp. 347-351, (2013)
[9] Choi E.H., Kang J.I., Cho J.Y., Et al., Supplementation of standardized lipid-soluble extract from maca (Lepidium meyenii) increases swimming endurance capacity in rats, Journal of Functional Foods, 4, 2, pp. 568-573, (2012)
[10] Zhang J., Wang H., Zhao Y., Et al., Comparison of mineral element content in a functional food maca(Lepidium meyenii Walp.) from Asia and South America, Journal of Analytical Methods in Chemistry, 2015, pp. 1-4, (2015)

← 1 2 →