The research on the material of hyaluronic acid, collagen and poly (ethylene glycol) diacrylate composite hydrogel

Aiming at the brittleness of hyaluronic acid (HA) and collagen (Col) hydrogels in mechanical properties, this work was to prepare a composite hydrogel with biocompatibility and high strength by two-step cross-linking method. Hydrogels with different weight percent of HA and constant Col and poly (ethylene glycol) Diacrylate were obtained to tune the physical and chemical properties. Subsequently, L-lysine was used to modify the hydrogels. The SEM results show the honeycomb morphology and the pore size of the ternary hydrogel ranges from 80-180 μm. When the concentration of L-lysine is 40 mmol/L, the comperssion modulus reaches 414 kPa which is 19 times of non-modified hydrogels. Cell toxicity and implantation experiments indicate that the extracts of hydrogel has no obvious inhibitory effect on MC 3T3-E1 cells, and the slight immunological rejection on mice. This kind of composite hydrogel has great potential in cartilage repair. ©, 2015, Journal of Functional Materials. All right reserved.