Effect of LuxS / AI-2 Quorum Sensing System on Resistance of Lactobacillus plantarum to Environmental Stress

被引：0
|
作者：
Ma J. [1 ]
Tan Z. [1 ]
Tian Z. [1 ]
Wu M. [1 ]
Wei X. [1 ]
Ren J. [1 ]
Jiang Z. [1 ]
Yu W. [1 ]
Hou J. [1 ]
机构：
[1] College of Food Science, Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin
来源：
Journal of Food Science and Technology (China) | 2024年 / 42卷 / 01期
基金：
中国国家自然科学基金; 黑龙江省自然科学基金;
关键词：
autoinducer-2; environmental stress; Lactobacillus plantarum; quorum sensing; stress response;
D O I：
10.12301/spxb202300211
中图分类号：
学科分类号：
摘要：
The LuxS/ AI-2 quorum sensing (QS) system mediates intraspecific and interspecific signals of lactic acid bacteria, among which autoinducer-2 (AI-2) is crucial for probiotic activities of lactic acid bacteria, including environmental stress tolerance, adhesion and colonization ability. However, the regulatory effect of LuxS/ AI-2 QS system in the resistance of Lactobacillus plantarum to various environmental stresses still needs to be systematically studied. The production of the signaling molecule AI-2 and the transcription of QS key genes luxS and pfs of Lactobacillus plantarum KLDS 1. 032 8 under environmental stress were analyzed by biological study and real-time fluorescence quantitative polymerase chain reaction. The results showed that acid stress could induce the production of signal molecule AI-2 and the transcription of luxS and pfs was significantly promoted under strong acid and alkali stress (P < 0. 05). Low temperature 25 ℃, high temperature 50 ℃ and hyperosmotic stress induced by mass fraction 6. 0% NaCl and mass fraction 3. 0% NaCl +3. 0% KCl could significantly inhibit cell proliferation, acid production and signal molecule AI-2 production. The transcription of luxS and pfs was up-regulated under low temperature stress at 25 ℃. The transcription of luxS and pfs was up-regulated with the increase of hypertonic stress. Furthermore, L. plantarum KLDS 1. 032 8 was induced to produce more signal molecule AI-2 by nutrient stress. With the aggravation of nutrient stress, the transcription of luxS was significantly up-regulated. The transcription of luxS and pfs reached the highest level when the nutrient volume fraction was 20%, while the transcription of pfs was significantly inhibited when the nutrient substance was diluted to volume fraction 40% -60% (P <0. 05). These results indicated that LuxS/ AI-2 QS system had different variation patterns under various environmental stresses, and played an important role in the stress resistance of L. plantarum KLDS 1. 032 8. © 2024 Beijing Technology and Business University, Department of Science and Technology. All rights reserved.
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页码：106 / 113
页数：7
相关论文
共 19 条
  • [1] LI J R, LI T T, WANG D F., Research progress on microbial quorum sensing system and its application in modern food industry [ J], Journal of Food Science and Technology, 38, 1, pp. 1-11, (2020)
  • [2] LI C, GAO M, ZHENG G S, Et al., Enhanced quorum sensing capacity via regulating microenvironment to facilitate stress resistance of probiotic in alginate-based micro-capsules, International Journal of Biological Macromolecules, 225, pp. 605-614, (2023)
  • [3] GU Y, LI B, TIAN J J, Et al., The response of LuxS/ AI-2 quorum sensing in Lactobacillus fermentum 2-1 to changes in environmental growth conditions, Annals of Microbiology, 68, 5, pp. 287-294, (2018)
  • [4] MUKHERJEE S, BASSLER B L., Bacterial quorum sensing in complex and dynamically changing environments [ J ], Nature Reviews Microbiology, 17, 6, pp. 371-382, (2019)
  • [5] GU Y, TIAN J J, ZHANG Y, Et al., Dissecting signal molecule AI-2 mediated biofilm formation and environmental tolerance in Lactobacillus plantarum, Journal of Bioscience and Bioengineering, 131, 2, pp. 153-160, (2021)
  • [6] MA J G, LI T Z, WANG Q Y, Et al., Enhanced viability of probiotics encapsulated within synthetic / natural bio-polymers by the addition of gum Arabic via electrohydrodynamic processing [ J ], Food Chemistry, 413, (2023)
  • [7] MA J G, XU C, LIU F, Et al., Stress adaptation and cross-protection of Lactobacillus plantarum KLDS 1. 0628, CyTA-Journal of Food, 19, 1, pp. 72-80, (2021)
  • [8] QIAN Y L, ZHAO C C, CAI X Y, Et al., Enhancing the AI-2 / LuxS quorum sensing system in Lactiplantibacillus plantarum: effect on the elimination of biofilms grown on seafoods, International Journal of Food Microbiology, 389, (2023)
  • [9] MALDONADO-BARRAGAN A, WEST S A., The cost and benefit of quorum sensing-controlled bacteriocin production in Lactobacillus plantarum, Journal of Evolutionary Biology, 33, 1, pp. 101-111, (2020)
  • [10] MA Q W, CHAI Y M, YANG Z B, Et al., Deciphering the mechanisms of Limosilactobacillus fermentum L1 involved in conjugated linoleic acid regulated by LuxS/ AI-2 quorum sensing, LWT-Food Science and Technology, 154, (2022)
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