Optimization of Cellulase Extraction Process of Crude Polysaccharide from Semen Cassiae and Study on Its Antioxidant Activity

被引：0

作者：

Jiang D. ^{[1
]}

Xu Z. ^{[1
]}

Luo Y. ^{[1
]}

Li Z. ^{[1
]}

Xiao J. ^{[1
]}

Mo Y. ^{[1
]}

Li Y. ^{[1
]}

Jiang L. ^{[1
]}

Chen X. ^{[1
]}

机构：

[1] College of Biology and Pharmacy, Guangxi Education Department Key Laboratory for Conservation and Utilization of Subtropical Bio-resources, Guangxi Key Laboratory of Agricultural Resources Chemistry and Biotechnology, Yulin Normal University, Yulin

来源：

Science and Technology of Food Industry | 2023年 / 44卷 / 09期

关键词：

antioxidant activity; cellulose; crude polysaccharide; response surface methodology; Semen Cassia;

D O I：

10.13386/j.issn1002-0306.2022060262

中图分类号：

学科分类号：

摘要：

Objective: To optimize the cellulase extraction process of crude polysaccharide from Semen Cassiae and study its antioxidant activity in vitro. Methods: On the basis of single factor test results, Box-Behnken response surface methodology was used to optimize the extraction process of crude polysaccharide from Semen Cassiae with enzymolysis time, enzymolysis temperature, enzyme dosage, liquid-to-material ratio and enzymolysis pH as independent variables, and the yield of polysaccharide as response value. DPPH radical and hydroxyl radical scavenging rates were used to investigate the antioxidant activities of crude polysaccharides from Semen Cassiae in vitro. Results: The optimal cellulase extraction conditions of crude polysaccharide from Semen Cassiae were as follows: Enzyme dosage was 1.4%, enzymolysis time was 50 min, liquid-to-material ratio was 24:1 mL/g, enzymolysis pH was 5.4, and enzymolysis temperature was 48 ℃. Under these conditions, the yield of polysaccharide from Semen Cassiae was 11.67%, and the theoretical prediction value of regression model was 11.91%, the error between the two conditions was less than 5%. Crude polysaccharide from Semen Cassiae had strong scavenging effects on DPPH free radical and hydroxyl free radical, with median inhibitory concentrations of 1.025 mg/mL and 0.894 mg/mL, respectively. Conclusion: Cellulase enzymatic method can significantly improve the yield of crude polysaccharide from Semen Cassiae, the process is simple and feasible, and the crude polysaccharide obtained from Semen Cassiae has antioxidant activity in vitro. © Science and Technology of Food Industry 2023.

引用

页码：183 / 189

页数：6

共 32 条

[1] YU F, SUN L, XU L J, Et al., Research progress on modern application of Cassiae semen[J], Modern Chinese Medicine, 20, 5, pp. 626-630, (2018)
[2] DONG Y J, JIANG Y Q, LIU Y, Et al., Research progress on chemical composition and pharmacological effects of Cassiae semen and predictive analysis on quality markers[J], Chinese Traditional and Herbal Drugs, 52, 9, pp. 2719-2732, (2021)
[3] ZHANG M, LI X, LIANG H, Et al., Semen Cassiae extract improves glucose metabolism by promoting GlUT4 translocation in the skeletal muscle of diabetic rats[J], Frontiers in Pharmacology, 9, (2018)
[4] LEE J Y, LIAO W L, LIU Y H, Et al., Oral administration of processed Cassia obtusifolia L. seed powder may reduce body weight and cholesterol in overweight patients with schizophrenia: A 36-week randomized, double-blind, controlled trial of high and low doses[J], Journal of Ethnopharmacology, 292, (2022)
[5] ZHU Z L, ZHANG S X, ZHENG Y Y, Et al., Effects of extract from Cassiae semen on blood lipid and liver and kidney function in hyperlipidemia rats[J], Preventive Medicine, 33, 12, pp. 1290-1294, (2021)
[6] GAO X M, ZHAO Q., Application of network pharmacology to predict the mechanism of Semen cassiae in the treatment of hypertension[J], Journal of Dali University, 6, 8, (2021)
[7] WANG Q Y, ZHOU J W, XIANG Z N, Et al., Anti-diabetic and renoprotective effects of Cassiae semen extract in the streptozotocin-induced diabetic rats[J], Journal of Ethnopharmacology, 239, (2019)
[8] WANG Q Y, TONG A H, PAN Y Y, Et al., The effect of Cassia seed extract on the regulation of the LKB1-AMPK-GLUT4 signaling pathway in the skeletal muscle of diabetic rats to improve the insulin sensitivity of the skeletal muscle[J], Diabetology Metabolic Syndrome, 11, 1, (2019)
[9] LUO H Y, WU H W, WANG L X, Et al., Hepatoprotective effects of Cassiae semen on mice with non-alcoholic fatty liver disease based on gut microbiota[J], Communications Biology, 4, 1, (2021)
[10] HE S Q, MA X, MENG Q G, Et al., Effects and mechanisms of water-soluble Semen Cassiae polysaccharide on retinitis pigmentosa in rats[J], Food Science and Technology, 40, 1, pp. 84-88, (2020)

← 1 2 3 4 →