Peptide sequence determination of peptidosteroids on a single bead by electrospray ionization-mass spectroscopy

被引：0
|
作者：
Vanhoenacker, Gerd [1 ]
Liu, Li [1 ]
Lynen, Frederic [1 ]
Madder, Annemieke [1 ]
De Clercq, Pierre [1 ]
Sandra, Pat [1 ]
机构：
[1] Ghent University, Faculty of Sciences, Department of Organic Chemistry, Krijgslaan 281 (S4), B-9000 Gent, Belgium
来源：
Journal of Separation Science | 2002年 / 25卷 / 10-11期
关键词：
High performance liquid chromatography - Ionization - Organic compounds;
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学科分类号：
摘要：
An electrospray ionization mass spectroscopy (ESI-MSn) method has been developed to simultaneously determine the sequence of two strands of tripeptides attached to a steroid-like scaffold. The peptidosteroid compounds were synthesized by solid-phase chemistry on TentaGel beads. The sequence of both peptide strands of a compound originating from a single bead could be determined. High-performance liquid chromatography hyphenated to MSn was also applied to a mixture of two isomeric peptidosteroids for sequence determination of their peptide chains.
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页码：671 / 676
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