Analysis of the Bovine Milk Fat Globule Membrane Protein Based on NanoLC-Orbitrap MS Technology

被引：0
|
作者：
Jing M. [1 ,2 ]
Zhang D. [2 ]
Jiang T. [2 ]
Wei J. [1 ,2 ]
Zhang Y. [2 ]
Liu B. [2 ]
Chen L. [1 ,2 ]
机构：
[1] School of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, Liaoning
[2] Beijing Maternal and Child Dairy Biotechnology Engineering Laboratory of Beijing Sanyuan Foods Co. Ltd., National Engineering Center of Dairy for Early Life Health, Beijing
来源：
Journal of Chinese Institute of Food Science and Technology | 2018年 / 18卷 / 04期
关键词：
Enzymatic method with super filter assisted; Milk fat globule membrane protein; Nanoflow liquid chromatography-orbitrap mass spectrometry (NanoLC-Orbitrap); Proteomics technology; Ultra speed centrifugal extraction;
D O I：
10.16429/j.1009-7848.2018.04.032
中图分类号：
学科分类号：
摘要：
A method for identification and quantitation of bovine milk fat globule membrane protein (MFGM) by using Nano LC-Orbitrap MS. MFGM were extracted from bovine milk by using ultra centrifugation method, followed by enzymatic hydrolysis with the help of super filteration, then the digested peptides were analysed by Nano LC-Orbitrap MS. Proteins were identified by searching Uniprot database. Gene Ontology (GO) annotation and classification was performed by mapping proteins (unique peptide ≥2) list to PANTHER classification system. In this application, the repeatability and accuracy of the method were verified by two techniques and three times. The extracted ion chromatogram of unique peptides and the MS/MS spectrometry of MFGM protein peptides showed that the high reliability and sensitivity. A total of 658 proteins were identified by one analysis. The data of 611 kinds of proteins were selected for analysis, among them, 593 proteins could be quantified (abundance>0), and 244 proteins had unique peptides numbers larger than 2. GO analysis of these 244 proteins showed that they were mainly distributed in cell membrane, and some molecular complex, and involved in biological pathways such as metabolic processes, cellular processes, cellular processes, biological regulation and immune system. These proteins also participated in many molecular functions, such as catalytic activity, binding activity, transport activity, enzyme regulation activity and structure activity. This method provided basics for future study of MFGM constitutions and functions by mass spectrometry. © 2018, Editorial Office of Journal of CIFST. All right reserved.
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页码：244 / 252
页数：8
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