A Novel Fluorescent Chemosensor for α-Amino Acids and Its Application for the Detection of Protease

被引：0

作者：

Cheng X. ^{[1
]}

Qu S. ^{[2
]}

Zhong Z. ^{[1
]}

机构：

[1] Hubei Key Laboratory of Low Dimensional Optoelectronic Materials and Devices, Hubei University of Arts and Science, Xiangyang

[2] School of Physics and Electronic Engineering, Hubei University of Arts and Science, Xiangyang

来源：

Cailiao Daobao/Materials Review | 2018年 / 32卷 / 07期

关键词：

Displacement approach; Fluorescence enhancement; Protease; Solubility; Α-amino acids;

D O I：

10.11896/j.issn.1005-023X.2018.14.029

中图分类号：

学科分类号：

摘要：

Complex C1-Cu(Ⅱ) could act as a new displacement-based fluorescent probe for α-amino acids detection. Upon the addition of α-amino acids, it displayed marked changes from non-fluorescence to strongly green fluorescence which could be observed by naked-eyes under a normal UV lamp. Especially, histidine exhibited the highest sensitivity: the fluorescent intensity increased to ~135-fold of the original one with the addition of excess histidine. Moreover, the intensity change at 490 nm was almost linear with the concentrations of histidine. Furthermore, as the hydrolysis of bovine serum albumin (BSA) with the aid of trypsin produces α-amino acids, the complex of C1-Cu(Ⅱ) with BSA could serve as a label-free, selective, fluorescent sensor toward trypsin with "turn-on" fluorescent methods. © 2018, Materials Review Magazine. All right reserved.

引用

页码：2486 / 2490

页数：4

共 40 条

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