In vitro clonal propagation of bird eye chilli (Capsicum frutescens Mill.)

被引：0
|
作者：
Gururaj, H.B. [1 ]
Giridhar, P. [1 ]
Sharma, Ashwani [1 ]
Prasad, B.C.N. [1 ]
Ravishankar, G.A. [1 ]
机构：
[1] Plant Cell Biotechnology Department, Ctrl. Food Technol. Res. Institute, Mysore 570 020, India
来源：
Indian Journal of Experimental Biology | 2004年 / 42卷 / 11期
关键词：
Activated carbon - Aromatic compounds - Cloning - Plants (botany);
D O I：
暂无
中图分类号：
学科分类号：
摘要：
An efficient and highly reproducible protocol for micropropagation of bird eye chilli Capsicum frutescens was attempted. Murashige and Skoog (MS) medium containing 0.5-3.0 mgl-1 of 6-benzyladenine (BA), 2-isopentenyl adenine (2iP), kinetin and 0.5-2.0 mg l-1 of indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) along with 1 gl-1 activated charcoal (AC) were used for shoot regeneration from both shoot tip and nodal explants. Shoot tip explants (100%) grew well on medium containing 1 mgl -1 of kinetin and 1 mgl-1 of IBA. Shoot proliferation (1-3) from nodal explants was effective on this medium. The regenerated shoots with 4-7 nodes had further growth upon sub-culturing onto kinetin (1 mgl -1) and IBA (1 mgl-1) and rooted simultaneously. The rooted plants were transferred to pots after hardening under controlled conditions. The survival percentage in pots was 80-90%.
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页码：1136 / 1140
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