Three-dimensional cardiomyocyte-based biosensor with tissue engineering scaffold

被引：2

作者：

Wei X.-W. ^{[1
]}

Gao Q. ^{[2
]}

Su K.-Q. ^{[1
]}

Qin Z. ^{[1
]}

Pan Y.-X. ^{[1
]}

He Y. ^{[2
]}

Wang P. ^{[1
]}

机构：

[1] Key Laboratory for Biomedical Engineering of Education Ministry, Zhejiang University, Hangzhou

[2] State Key Laboratory of Fluid Power and Mechatronic Systems, Zhejiang University, Hangzhou

来源：

Wang, Ping (cnpwang@zju.edu.cn) | 2018年 / Zhejiang University卷 / 52期

关键词：

Cardiomyocyte; Extracellular field potential; Microelectrode array; Three-dimensional cell-based biosensor; Tissue engineering scaffold;

D O I：

10.3785/j.issn.1008-973X.2018.07.023

中图分类号：

学科分类号：

摘要：

Polylactic acid (PLA) and polycaprolactone (PCL) were selected as materials to fabricate tissue engineering scaffolds by three-dimensional (3D) printing and electrospinning, which were used to culture cardiomyocytes of neonatal rats. Then the scaffolds with cardiomyocytes were coupled with microelectrode array (MEA) to form a 3D cell-based biosensor, which was used to detect the extracellular field potential (EFP) of cardiomyocytes. The experimental results demonstrated that cardiomyocytes adhered and grew well in scaffolds, and could drive fibers to produce combined beating due to the excitation-contraction coupling. After 48 hours, the beating rate of cardiomyocytes in the scaffolds tended to be stable. The detecting results demonstrated that scaffolds and MEA were coupled well to be a 3D cell-based biosensor system, which could detect the EFP of cardiomyocytes in scaffolds with stable and high-SNR signals. The EFP amplitude and firing rate were both similar to the signals recorded from traditional two-dimensional (2D) culturing method. © 2018, Zhejiang University Press. All right reserved.

引用

页码：1415 / 1422

页数：7

共 28 条

[1] Langer R., Vacanti J.P., Tissue engineering, Science, 260, 5110, pp. 920-926, (1993)
[2] Sachlos E., Czernuszka J.T., Making tissue engineering scaffolds work. Review: the application of solid freeform fabrication technology to the production of tissue engineering scaffolds, European Cells and Materials, 5, 29, pp. 39-40, (2003)
[3] Nam Y.S., Park T.G., Porous biodegradable polymeric scaffolds prepared by thermally induced phase separation, Journal of Biomedical Materials Research, 47, 1, pp. 8-17, (1999)
[4] Intranuovo F., Gristina R., Brun F., Et al., Plasma modification of PCL porous scaffolds fabricated by solvent-casting/particulate-leaching for tissue engineering, Plasma Processes and Polymers, 11, 2, pp. 184-195, (2014)
[5] Wu X., Liu Y., Li X., Et al., Preparation of aligned porous gelatin scaffolds by unidirectional freeze-drying method, Acta Biomaterialia, 6, 3, pp. 1167-1177, (2010)
[6] Pham Q.P., Sharma U., Mikos A.G., Electrospinning of polymeric nanofibers for tissue engineering applications: a review, Tissue Engineering, 12, 5, pp. 1197-1211, (2006)
[7] Garrigues N.W., Little D., Sanchez-Adams J., Et al., Electrospun cartilage-derived matrix scaffolds for cartilage tissue engineering, Journal of Biomedical Materials Research Part A, 102, 11, pp. 3998-4008, (2014)
[8] Jang J.H., Castano O., Kim H.W., Electrospun materials as potential platforms for bone tissue engineering, Advanced Drug Delivery Reviews, 61, 12, pp. 1065-1083, (2009)
[9] Hasan A., Memic A., Annabi N., Et al., Electrospun scaffolds for tissue engineering of vascular grafts, Acta Biomaterialia, 10, 1, pp. 11-25, (2014)
[10] Zhao G., Zhang X., Lu T.J., Et al., Recent advances in electrospun nanofibrous scaffolds for cardiac tissue engineering, Advanced Functional Materials, 25, 36, pp. 5726-5738, (2015)

← 1 2 3 →