In-vitro effect of antimicrobial photodynamic therapy with methylene blue in two different genera of dermatophyte fungi

被引:0
|
作者
Spezzia-Mazzocco T. [1 ]
Torres-Hurtado S.A. [1 ]
Ramírez-San-Juan J.C. [1 ]
Ramos-García R. [1 ]
机构
[1] Departamento de Óptica, Instituto Nacional de Astrofísica, Óptica y Electrónica (INAOE), Luis Enrique Erro # 1, Tonantzintla, Puebla
来源
Photonics and Lasers in Medicine | 2016年 / 5卷 / 03期
关键词
dermatophytes; fungal infection; photodynamic therapy; photosensitizer;
D O I
10.1515/plm-2016-0021
中图分类号
学科分类号
摘要
Background and objectives: Antimicrobial photodynamic therapy (aPDT) is a technique that combines the photoactivation properties of an innocuous chromophore or photosensitizer (PS) and light, producing reactive oxygen molecules that trigger cell death processes. In this study the in-vitro application of aPDT to fight fungal infections was investigated using methylene blue (MB) as the PS. Materials and methods: The antimicrobial PDT process was carried out with MB and red laser light (λ = 633 nm) to activate the PS. Testing was performed with suspensions of various species of dermatophyte fungi (Trichophyton mentagrophytes, Microsporum canis and Microsporum gypseum), including a fungus, which to our knowledge, has not been previously studied using this dye (Trichophyton tonsurans). For T. tonsurans further optimization tests were carried out. Results and discussion: The fungicidal effect of MB-aPDT was evident. Microsporum strains were slightly more sensitivity to the treatment than Trichophyton strains. The response of T. tonsurans to aPDT was less than to the other fungi tested under the same conditions, or even with higher fluence. However, repetitive aPDT treatment with very low doses of light can achieve a good effectiveness with this strain effecting total growth inhibition. Light may even disturb fungi growth in some circumstances, especially in strain such as T. tonsurans. Conclusion: This study with Trichophyton and Microsporum strains showed that MB was an effective PS to inhibit fungal growth through aPDT, reaching a total inhibition in most of the fungi tested. It was found that repeated exposure with low-power light within the framework of aPDT treatment can achieve better results than a single exposure at higher power. © 2016 Walter de Gruyter GmbH, Berlin/Boston.
引用
收藏
页码:203 / 210
页数:7
相关论文
共 50 条
  • [1] Effect of Photodynamic Therapy with Methylene Blue on Acanthamoeba In Vitro
    Mito, Tsuyoshi
    Suzuki, Takashi
    Kobayashi, Takeshi
    Zheng, Xiaodong
    Hayashi, Yasuhito
    Shiraishi, Atsushi
    Ohashi, Yuichi
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2012, 53 (10) : 6305 - 6313
  • [2] In Vitro Evaluation of the Antibacterial Effect of Photodynamic Therapy with Methylene Blue
    Chaves de Vasconcelos Catao, Maria Helena
    Araujo Batista, Ana Luzia
    PESQUISA BRASILEIRA EM ODONTOPEDIATRIA E CLINICA INTEGRADA, 2020, 20 : 1 - 10
  • [3] Effect of Photodynamic Therapy with the Photosensitizer Methylene Blue on Cerebral Endotheliocytes In Vitro
    Makarov, Vladimir I.
    Skobeltsin, Alexey S.
    Averchuk, Anton S.
    Berdnikov, Arseniy K.
    Chinenkova, Milana V.
    Salmina, Alla B.
    Loschenov, Victor B.
    PHOTONICS, 2024, 11 (04)
  • [4] Methylene Blue Photodynamic Antimicrobial Therapy Inhibition Against Common Ocular Isolates In Vitro
    Chou, Brandon
    Krishna, Katherine
    Durkee, Heather Ann
    Echeverri, Felipe
    Lai, James
    Amescua, Guillermo
    Miller, Darlene
    Parel, Jean-Marie
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2023, 64 (08)
  • [5] Evaluation of the Effects of Photodynamic Therapy With Methylene Blue on Different Candida Species In Vitro
    Khozeimeh, Faezeh
    Tavangar, Atefeh
    Razzaghi-Abyaneh, Mehdi
    Amini, Milad
    Ghayour, Marzieh
    JOURNAL OF LASERS IN MEDICAL SCIENCES, 2023, 14
  • [6] Latex membranes with methylene blue dye for antimicrobial photodynamic therapy
    Maria Andrelina O. Sousa
    Marco A. C. de Faria
    Rita P. Ribeiro
    João V. P. Valverde
    Herica D. Rocha
    Kevin F. dos Santos
    Marcos S. Sousa
    Paula C. S. Souto
    Josmary R. Silva
    Nara C. de Souza
    Photochemical & Photobiological Sciences, 2021, 20 : 1027 - 1032
  • [7] Latex membranes with methylene blue dye for antimicrobial photodynamic therapy
    O. Sousa, Maria Andrelina
    de Faria, Marco A. C.
    Ribeiro, Rita P.
    Valverde, Joao V. P.
    Rocha, Herica D.
    dos Santos, Kevin F.
    Sousa, Marcos S.
    Souto, Paula C. S.
    Silva, Josmary R.
    de Souza, Nara C.
    PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, 2021, 20 (08) : 1027 - 1032
  • [8] In Vitro Antimicrobial Photodynamic Therapy Against Trichophyton mentagrophytes Using New Methylene Blue as the Photosensitizer
    Lopez-Chicon, P.
    Gulias, O.
    Nonell, S.
    Agut, M.
    ACTAS DERMO-SIFILIOGRAFICAS, 2016, 107 (09): : 765 - 770
  • [9] Effective Biofilm Eradication on Orthopedic Implants with Methylene Blue Based Antimicrobial Photodynamic Therapy In Vitro
    Prinz, Julia
    Wink, Marianne
    Neuhaus, Sonja
    Grob, Markus C.
    Walt, Heinrich
    Bosshard, Philipp P.
    Achermann, Yvonne
    ANTIBIOTICS-BASEL, 2023, 12 (01):
  • [10] Antimicrobial efficacy of methylene blue-mediated photodynamic therapy on titanium alloy surfaces in vitro
    Huang, Tsun-Chin
    Chen, Chun-Ju
    Ding, Shinn-Jyh
    Chen, Chun-Cheng
    PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY, 2019, 25 : 7 - 16